CTSC Kit ELISA

N° du produit ABIN5652694

Détail du produit CTSC Kit ELISA

Antigène: CTSC (Cathepsin C (CTSC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cathepsin C (CTSC). No significant cross-reactivity or interference between Cathepsin C (CTSC) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin C (CTSC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin C (CTSC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin C (CTSC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin C (CTSC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin C (CTSC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin C (CTSC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CTSC (Cathepsin C (CTSC))

Autre désignation: Cathepsin C (CTSC Produits)

Synonymes: CPPI Kit ELISA, DPP-I Kit ELISA, DPP1 Kit ELISA, DPPI Kit ELISA, HMS Kit ELISA, JP Kit ELISA, JPD Kit ELISA, PALS Kit ELISA, PDON1 Kit ELISA, PLS Kit ELISA, MGC69126 Kit ELISA, MGC107782 Kit ELISA, AI047818 Kit ELISA, CATC Kit ELISA, cb912 Kit ELISA, ik:tdsubc_1h2 Kit ELISA, sb:cb146 Kit ELISA, wu:fb34g12 Kit ELISA, wu:fj58d01 Kit ELISA, cathepsin C Kit ELISA, cathepsin C L homeolog Kit ELISA, cathepsin CPC1 Kit ELISA, CTSC Kit ELISA, ctsc.L Kit ELISA, ctsc Kit ELISA, TP03_0357 Kit ELISA, CMU_003360 Kit ELISA, CPC1 Kit ELISA, NAEGRDRAFT_80477 Kit ELISA, NAEGRDRAFT_37577 Kit ELISA, Ctsc Kit ELISA, Smp_183330 Kit ELISA

Sujet:

Gene Name: Cathepsin C

Gene Aliases: CTS-C, DPPI, CPPI, DPP1, DPP-I, HMS, JP, JPD, PALS, PLS, Dipeptidyl Peptidase I, Dipeptidyl transferase, Cathepsin J

ID gène: 1075

UniProt: P53634