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MSTN Kit ELISA

MSTN Reactivité: Humain Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5657111
  • Antigène Voir toutes MSTN Kits ELISA
    MSTN (Myostatin (MSTN))
    Reactivité
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    617.3 pg/mL - 50000 pg/mL
    Seuil minimal de détection
    617.3 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Myostatin (MSTN). No significant cross-reactivity or interference between Myostatin (MSTN) and analogues was observed.
    Sensibilité
    264.1 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Myostatin (MSTN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Myostatin (MSTN) and unlabeled Myostatin (MSTN) (Standards or samples) with the pre-coated antibody specific to Myostatin (MSTN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Myostatin (MSTN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Myostatin (MSTN) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myostatin (MSTN) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myostatin (MSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MSTN Kits ELISA
    MSTN (Myostatin (MSTN))
    Autre désignation
    Myostatin (MSTN Produits)
    Synonymes
    GDF-8 Kit ELISA, GDF8 Kit ELISA, gdf8 Kit ELISA, mstn Kit ELISA, zgc:109749 Kit ELISA, Gdf8 Kit ELISA, NV13143 Kit ELISA, MSLHP Kit ELISA, Cmpt Kit ELISA, myostatin Kit ELISA, myostatin b Kit ELISA, growth/differentiation factor 8 Kit ELISA, MSTN Kit ELISA, mstnb Kit ELISA, LOC5577632 Kit ELISA, BRAFLDRAFT_290840 Kit ELISA, Mstn Kit ELISA, mstn Kit ELISA
    Sujet

    Gene Name: Myostatin

    Gene Aliases: GDF8, Growth Differentiation Factor 8

    ID gène
    2660
    UniProt
    O14793
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