PTGDS Kit ELISA

N° du produit ABIN5658138

Détail du produit PTGDS Kit ELISA

Antigène: PTGDS (Prostaglandin D2 Synthase (PTGDS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cerebrospinal Fluid, Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Prostaglandin D2 Synthase, Brain (PGD2S). No significant cross-reactivity or interference between Prostaglandin D2 Synthase, Brain (PGD2S) and analogues was observed.

Sensibilité: 0.129 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin D2 Synthase, Brain (PGD2S). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin D2 Synthase, Brain (PGD2S). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin D2 Synthase, Brain (PGD2S), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin D2 Synthase, Brain (PGD2S) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin D2 Synthase, Brain (PGD2S) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin D2 Synthase, Brain (PGD2S) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PTGDS (Prostaglandin D2 Synthase (PTGDS))

Autre désignation: Prostaglandin D2 Synthase, Brain (PTGDS Produits)

Synonymes: 6PGD Kit ELISA, GSTS Kit ELISA, GSTS1-1 Kit ELISA, PGD2 Kit ELISA, PGDS Kit ELISA, L-PGDS Kit ELISA, LPGDS Kit ELISA, PDS Kit ELISA, PGDS2 Kit ELISA, H-PGDS Kit ELISA, Ptgds2 Kit ELISA, 21kDa Kit ELISA, Ptgs3 Kit ELISA, PH2DISO Kit ELISA, phosphogluconate dehydrogenase Kit ELISA, hematopoietic prostaglandin D synthase Kit ELISA, prostaglandin D2 synthase Kit ELISA, prostaglandin D2 synthase (brain) Kit ELISA, prostaglandin D synthase Kit ELISA, prostaglandin D2 synthase 21kDa (brain) Kit ELISA, PGD Kit ELISA, HPGDS Kit ELISA, PTGDS Kit ELISA, Hpgds Kit ELISA, Ptgds Kit ELISA, LOC100009453 Kit ELISA

Sujet:

Gene Name: Prostaglandin D2 Synthase, Brain

Gene Aliases: PTGDS, PDS, PGDS2, Prostaglandin-H2 D-Isomerase, Lipocalin-Type Prostaglandin D Synthase, Cerebrin-28, Beta-trace protein, Glutathione-independent PGD synthase

ID gène: 5730

UniProt: P41222