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BLUeye Prestained Protein Ladder

10-245 kDa Colorimetric
N° du produit ABIN2868252
  • Méthode de détection
    Colorimetric
    Gamme de detection
    10-245 kDa
    Seuil minimal de détection
    10 kDa
    Application
    SDS-PAGE (SDS), Western Blotting (WB)
  • Indications d'application
    Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
    BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
    Commentaires

    The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    Préparation des réactifs

    Quality Control:
    Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Bis-Tris 10 % Tris-Glycine Bis-Tris 10 % MES buffer 4~20 % MOPS buffer
    Stock
    RT
    Stockage commentaire
    Store at 25°C for 6 months, Store at -20°C for 24 months
    Date de péremption
    24 months
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