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Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) Peptide

PIN1 Reactivité: Humain Hôte: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
N° du produit ABIN934902
  • Antigène Tous les produits PIN1
    PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Protein Region
    AA 22-255, Extracellular Domain
    Origine
    Humain
    Source
    • 7
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Séquence
    MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
    Attributs du produit
    Purified recombinant Human Pin 1 protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 162 nM/min/µg, and is defined as the amount of enzyme that cleaves 1 uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
    Molecular weight on SDS-PAGE will appear higher.
    Pureté
    > 95 % pure
    niveau d'endotoxine
    < 1.0 EU per µg of protein (determined by LAL method)
  • Indications d'application
    Pin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Procédure de l'essai
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant Pin 1 protein at various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH7.5, containing 0.1 M NaCl, 0 mM DTT, and 20 % glycerol.
    Agent conservateur
    Dithiothreitol (DTT)
    Précaution d'utilisation
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    RT/-20 °C
    Stockage commentaire
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Antigène
    PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
    Synonymes
    DOD Peptide, UBL5 Peptide, dod Peptide, ubl5 Peptide, pin1 Peptide, pin1a Peptide, 0610025L01Rik Peptide, D9Bwg1161e Peptide, pin1b Peptide, zgc:73206 Peptide, PPIase pin1 Peptide, MdPin1 Peptide, Pin1 Peptide, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 Peptide, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 S homeolog Peptide, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 Peptide, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 L homeolog Peptide, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Peptide, peptidyl-prolyl cis-trans isomerase Pin1 Peptide, PIN1 Peptide, Pin1 Peptide, pin1 Peptide, pin1.S Peptide, pin1.L Peptide, ppiD Peptide, PITG_11123 Peptide, LOC103419188 Peptide
    Sujet
    Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
    Alternative Names: Rotamase Pin1 protein, UBL5 protein, PPIase protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein, Pin -1 protein, Pin -1, Pin 1, PPIase Pin1 protein, Pin 1 protein, PPIase Pin1 protein, Rotamase Pin1 protein, DOD protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, PIN1 protein, PIN1 protein, EC 5.2.1.8 protein, Pin 1, EC 5.2.1.8 protein
    Poids moléculaire
    18.2 kDa (163 AA)
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