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SMG7 Protein (AA 2-1137) (His tag)

Crystallography grade SMG7 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, Crys, ELISA, WB
N° du produit ABIN3095443
  • Antigène Voir toutes SMG7 Protéines
    SMG7 (Smg-7 Homolog, Nonsense Mediated mRNA Decay Factor (SMG7))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-1137
    Origine
    Humain
    Source
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette SMG7 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Crystallization (Crys), ELISA, Western Blotting (WB)
    Séquence
    SLQSAQYLRQ AEVLKADMTD SKLGPAEVWT SRQALQDLYQ KMLVTDLEYA LDKKVEQDLW NHAFKNQITT LQGQAKNRAN PNRSEVQANL SLFLEAASGF YTQLLQELCT VFNVDLPCRV KSSQLGIISN KQTHTSAIVK PQSSSCSYIC QHCLVHLGDI ARYRNQTSQA ESYYRHAAQL VPSNGQPYNQ LAILASSKGD HLTTIFYYCR SIAVKFPFPA ASTNLQKALS KALESRDEVK TKWGVSDFIK AFIKFHGHVY LSKSLEKLSP LREKLEEQFK RLLFQKAFNS QQLVHVTVIN LFQLHHLRDF SNETEQHTYS QDEQLCWTQL LALFMSFLGI LCKCPLQNES QEESYNAYPL PAVKVSMDWL RLRPRVFQEA VVDERQYIWP WLISLLNSFH PHEEDLSSIS ATPLPEEFEL QGFLALRPSF RNLDFSKGHQ GITGDKEGQQ RRIRQQRLIS IGKWIADNQP RLIQCENEVG KLLFITEIPE LILEDPSEAK ENLILQETSV IESLAADGSP GLKSVLSTSR NLSNNCDTGE KPVVTFKENI KTREVNRDQG RSFPPKEVRR DYSKGITVTK NDGKKDNNKR KTETKKCTLE KLQETGKQNV AVQVKSQTEL RKTPVSEARK TPVTQTPTQA SNSQFIPIHH PGAFPPLPSR PGFPPPTYVI PPPVAFSMGS GYTFPAGVSV PGTFLQPTAH SPAGNQVQAG KQSHIPYSQQ RPSGPGPMNQ GPQQSQPPSQ QPLTSLPAQP TAQSTSQLQV QALTQQQQSP TKAVPALGKS PPHHSGFQQY QQADASKQLW NPPQVQGPLG KIMPVKQPYY LQTQDPIKLF EPSLQPPVMQ QQPLEKKMKP FPMEPYNHNP SEVKVPEFYW DSSYSMADNR SVMAQQANID RRGKRSPGVF RPEQDPVPRM PFEKSLLEKP SELMSHSSSF LSLTGFSLNQ ERYPNNSMFN EVYGKNLTSS SKAELSPSMA PQETSLYSLF EGTPWSPSLP ASSDHSTPAS QSPHSSNPSS LPSSPPTHNH NSVPFSNFGP IGTPDNRDRR TADRWKTDKP AMGGFGIDYL SATSSSESSW HQASTPSGTW TGHGPSMEDS SAVLMESLKS IWSSSMMHPG PSALEQLLMQ QKQKQQRGQG TMNPPH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SMG7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    SMG7 (Smg-7 Homolog, Nonsense Mediated mRNA Decay Factor (SMG7))
    Autre désignation
    SMG7 (SMG7 Produits)
    Synonymes
    9430023P16Rik Protein, mKIAA0250 Protein, C1orf16 Protein, EST1C Protein, SGA56M Protein, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) Protein, SMG7, nonsense mediated mRNA decay factor Protein, Smg7 Protein, SMG7 Protein
    Sujet
    Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. {ECO:0000269|PubMed:15546618, ECO:0000269|PubMed:15721257}.
    Poids moléculaire
    128.1 kDa Including tag.
    UniProt
    Q92540
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