Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

ST5 Protein (AA 1-1137) (His tag)

Crystallography grade ST5 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3095650
  • Antigène Voir toutes ST5 Protéines
    ST5 (Suppression of Tumorigenicity 5 (ST5))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1137
    Origine
    • 2
    • 1
    Humain
    Source
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette ST5 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MTMTANKNSS ITHGAGGTKA PRGTLSRSQS VSPPPVLSPP RSPIYPLSDS ETSACRYPSH SSSRVLLKDR HPPAPSPQNP QDPSPDTSPP TCPFKTASFG YLDRSPSACK RDAQKESVQG AAQDVAGVAA CLPLAQSTPF PGPAAGPRGV LLTRTGTRAH SLGIREKISA WEGRREASPR MSMCGEKREG SGSEWAASEG CPSLGCPSVV PSPCSSEKTF DFKGLRRMSR TFSECSYPET EEEGEALPVR DSFYRLEKRL GRSEPSAFLR GHGSRKESSA VLSRIQKIEQ VLKEQPGRGL PQLPSSCYSV DRGKRKTGTL GSLEEPAGGA SVSAGSRAVG VAGVAGEAGP PPEREGSGST KPGTPGNSPS SQRLPSKSSL DPAVNPVPKP KRTFEYEADK NPKSKPSNGL PPSPTPAAPP PLPSTPAPPV TRRPKKDMRG HRKSQSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ST5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ST5 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ST5 (Suppression of Tumorigenicity 5 (ST5))
    Autre désignation
    ST5 (ST5 Produits)
    Synonymes
    zgc:91902 Protein, wu:fc11b09 Protein, si:ch211-208c6.1 Protein, ST5 Protein, DKFZp459D1230 Protein, hts1 Protein, p126 Protein, dennd2b Protein, DENND2B Protein, HTS1 Protein, 2010004M01Rik Protein, 2610305K15Rik Protein, suppression of tumorigenicity 5 Protein, st5 Protein, ST5 Protein, St5 Protein
    Sujet
    Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth. {ECO:0000269|PubMed:10229203, ECO:0000269|PubMed:20937701, ECO:0000269|PubMed:9632734}.
    Poids moléculaire
    127.4 kDa Including tag.
    UniProt
    P78524
Vous êtes ici:
Support technique