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RFWD3 Protein (AA 1-774) (His tag)

Crystallography grade RFWD3 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3136488
  • Antigène Voir toutes RFWD3 Protéines
    RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-774
    Origine
    Souris
    Source
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette RFWD3 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MAEVAVGQAS DLVPSEMDHE VIYSHLQGPL EGTIEPATPT EVVSNGAPLQ PAPAELANSQ GGAHVQPAPA EVVSSQDGLP TLQPLPPASI DLTEEVQPSE ENMEVVNPGT SEEPSQGSGA NPTAGAARSV SMNNFISGLQ RLHNMLELLR PPPADHSVGP IRTRRRMAPI LRARAGESQR QDNGRYVPHT PLYAYFQVSR SQPYPLPAAH DSETRNPPSG TDSDSDGSAE DEEVVVQAEE PEANIPEQGV IATDQEATSV TGDDAVPKES PQKPNMLSAM EDEEGETCTI CLEQWTNAGD HRISALRCGH LFGFRCISKW LKGQTRKCPQ CNKKAKHSDI VVIYARSLRA LDTSEQERMK SDLLNEQMLR KQAELESAQC RLQLQVLIDK CTKLNSRVQD LEKFVVRHQN SAAQQCSRSK ALCGKCLSSS QSPGKYSSEK TFTISQTGKC RILAYCDGLS CLVASQPSPQ ASFLPGFGVK MLSTANMKSS QYIPMHNKQI RGLSFSSQSK GLLLSASLDC TIKLTSLETN TVVQTYNTGR PVWSCCWCLD ENNYVYAGLA SGSILIYDLR NTNTYIQELV PQKARCPLVS LSYLPKAAVA AFPYGGVLAG TLENASFWEL KSDFSHKPHV LSLEPGGFVD FQTESSTRHC LVTYRPDKSH NTVRTVLLEM SYKLDDAGEP VCSCLPVQTF LGGPTCKLLT KSAIFQSPEN NGNVLVCTGD EASQSTLLWN AGSGLLLQNL KAGEPVLDIC PFETNQNSYL ATLTEKTVHM YKWE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Rfwd3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product RFWD3 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))
    Autre désignation
    Rfwd3 (RFWD3 Produits)
    Synonymes
    RGD1305243 Protein, RFWD3 Protein, RNF201 Protein, BC027246 Protein, ring finger and WD repeat domain 3 Protein, Rfwd3 Protein, RFWD3 Protein
    Sujet
    E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair (By similarity). {ECO:0000250}.
    Poids moléculaire
    85.3 kDa Including tag.
    UniProt
    Q8CIK8
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