Checkpoint Kinase 1 (CHEK1) (Active) Protéine

Détails pour le produit réf. ABIN457519, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Origine
Humain
14
3
1
1
1
Source
Baculovirus infected Insect Cells
7
4
3
2
1
1
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Fonction >90% Pure active Recombinant CHK1.
Attributs du produit Protein Source: Baculovirus (Sf9 insect cells)
Pureté SDS-PAGE: ≥90 %
Sujet CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.
Alternate Names: CHK, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1
Poids moléculaire 82.0 kDa
ID gène 1111
UniProt O14757
Domaine de recherche Kinases/Phosphatases, Cell Cycle
Pathways Signalisation p53, Apoptose, Cycle Cellulaire
Indications d'application Optimal working dilution should be determined by the investigator.
Commentaires

Activity Specification: 369 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30 % glycerol.).
Agent conservateur Dithiothreitol (DTT)
Précaution d'utilisation This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Centrifuge the vial prior to opening.
Stock -80 °C
Stockage commentaire -80°C
Date de péremption 12 months
Supplier Images
Western Blotting (WB) image for Checkpoint Kinase 1 (CHEK1) (Active) Protéine (ABIN457519) Checkpoint Kinase 1 (CHEK1) (Active) protein
Produit citée dans: Larsen, Rampalli, Burns, Brunette, Dilworth, Megeney: "Caspase 3/caspase-activated DNase promote cell differentiation by inducing DNA strand breaks." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 9, pp. 4230-5, 2010 (PubMed).