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IL12A & IL27B (AA 20-189) (Active) protein (His tag,AVI tag,Biotin)

Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
N° du produit ABIN4949030
  • Antigène
    IL12A & IL27B
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 20-189
    Origine
    • 4
    • 3
    • 1
    • 1
    Humain
    Source
    • 5
    • 1
    HEK-293 Cells
    Purification/Conjugué
    His tag,AVI tag,Biotin
    Application
    Functional Studies (Func)
    Marque
    MABSol®,PrecisionAvi
    Séquence
    AA 20-189
    Specificité
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Attributs du produit
    Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.
    Pureté
    >95 % as determined by SDS-PAGE.
    niveau d'endotoxine
    Less than 1.0 EU per μg by the LAL method.
  • Commentaires

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Conseil sur la manipulation
    Please avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
  • Antigène
    IL12A & IL27B
    Autre désignation
    IL23A & IL12B
    Sujet
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
    Poids moléculaire
    21.9 kDa (IL23A) & 34.7 kDa (IL12B)
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