HRAS Protein (AA 1-185) (His tag)

N° du produit ABIN667031

Cette protéine Recombinant HRAS est produite dans Escherichia coli (E. coli).

Aperçu rapide pour HRAS Protein (AA 1-185) (His tag) (ABIN667031)

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 1-185

Purification/Conjugué: Cette HRAS protéine est marqué à la His tag.

Séquence: MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG HEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKC

Attributs du produit: KRAS, 1-185aa, Human, His tag, E.coli

Purification: > 90% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl,1 mM DTT, 10 % glycerol

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Autre désignation: K-Ras

Sujet: KRAS is a member of the small GTPase superfamily. This protein is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. under normal conditions, Ras family members influence cell growth and differentiation events in a subcellular membrane compartmentalization-based signaling system. Oncogenic Ras can deregulate processes that control both cell proliferation and apoptosis. Recombinant human KRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 23.2 kDa (205aa) confirmed by MALDI-TOF

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling