Rat (Rattus) EPH Receptor A5 ELISA Kit

Recommended EPH Receptor A5 Product (fourni par: Connectez-vous pour afficher )

Antigène
EPH Receptor A5 (EPHA5) Kits ELISA
  • CEK7
  • EHK-1
  • EHK1
  • EK7
  • HEK7
  • TYRO4
  • AI854630
  • AW125296
  • Cek7
  • Ehk1
  • Els1
  • Hek7
  • Rek7
  • bsk
  • Els1.
  • EPHA5
  • EPH receptor A5
  • Eph receptor A5
  • EPHA5
  • epha5
  • Epha5
Reactivité
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
4
3
3
Type de méthode
Sandwich ELISA
Application
ELISA
Options
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N° du produit ABIN2748078
$ 734.80
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Fournisseur References Details
1 ABIN1381109 Cell ELISA Cell Culture Cells Connectez-vous pour afficher
1 ABIN2113775 Cell ELISA Cell Culture Cells Connectez-vous pour afficher
1 ABIN2684129 Cell ELISA Cell Samples, Suspension Cell Culture Connectez-vous pour afficher

General

Antigène EPH Receptor A5 (EPHA5) Kits ELISA
Reactivité Rat (Rattus)
Kits with alternative reactivity to:
(4), (3), (3)
Type de méthode Sandwich ELISA
Application ELISA
Fournisseur Connectez-vous pour afficher

Détail du produit EPH Receptor A5 ELISA Kit

Détail du antigène Information d'application Stockage Images
Fonction Custom Rat EphA5 ELISA Kit.
Type d'échantillon Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Méthode de détection Colorimetric
Analytical Method Quantitative
Specificité The antibody pair provided in this kit recognizes Rat EphA5
Attributs du produit
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Détail du antigène

Détail du produit EPH Receptor A5 ELISA Kit Information d'application Stockage Images Haut de la page
Antigène
Autre désignation EphA5 (EPHA5 ELISA Kit Extrait)
Sujet EphA5
ID gène 79208
UniProt P54757
Pathways Signalisation RTK

Information d'application

Détail du produit EPH Receptor A5 ELISA Kit Détail du antigène Stockage Images Haut de la page
Volume d'échantillon 100 μL
Plaque Pre-coated
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Procédure de l'essai Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Stockage

Détail du produit EPH Receptor A5 ELISA Kit Détail du antigène Information d'application Images Haut de la page
Stock -20 °C
Stockage commentaire The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption 6 months