Souris EPO ELISA Kit

Recommended EPO Product (fourni par: Connectez-vous pour afficher )

Antigène
Erythropoietin (EPO) Kits ELISA
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • EPO
  • epo
  • Epo
Épitope
AA 27-192
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
21
20
20
12
9
9
6
5
5
4
3
3
2
2
1
1
Type de méthode
Sandwich ELISA
Gamme de detection
46.9-3000 pg/mL
Seuil minimal de détection
46.9 pg/mL
Application
ELISA
Options
Fournisseur
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N° du produit ABIN921054
539,52 €
Plus frais de livraison 40,00 € et TVA
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Fournisseur References Details
12.499185 ABIN415364 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Connectez-vous pour afficher
12.499185 ABIN366328 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Connectez-vous pour afficher
12.499185 ABIN1114810 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Connectez-vous pour afficher
12.499185 ABIN491741 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 1.37-1.000 pg/mL 1.37 pg/mL Connectez-vous pour afficher
12.499185 ABIN4885513 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 1.5-400 pg/mL 1.5 pg/mL Connectez-vous pour afficher
12.499185 ABIN2708505 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Connectez-vous pour afficher
12.499185 ABIN2113847 Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate Connectez-vous pour afficher
1 ABIN1569609 Sandwich ELISA Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Connectez-vous pour afficher
1 ABIN773070 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Connectez-vous pour afficher
1 ABIN4947538 Sandwich ELISA Connectez-vous pour afficher
1 ABIN5593376 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Connectez-vous pour afficher
1 ABIN2684142 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Connectez-vous pour afficher 1
1 ABIN5522730 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 47-3000 pg/mL 47 pg/mL Connectez-vous pour afficher
1 ABIN2042456 Sandwich ELISA 46.9-3000 pg/mL 46.9 pg/mL Connectez-vous pour afficher
1 ABIN2533111 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Connectez-vous pour afficher
1 ABIN4968934 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Connectez-vous pour afficher
1 ABIN455063 78-5000 pg/mL 78 pg/mL Connectez-vous pour afficher
1 ABIN2944089 1.37-1000 pg/mL 1.37 pg/mL Connectez-vous pour afficher
1 ABIN2947313 0.156-10 ng/mL 0.156 ng/mL Connectez-vous pour afficher
1 ABIN5072547 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Connectez-vous pour afficher

General

Antigène Erythropoietin (EPO) Kits ELISA
Épitope AA 27-192
Reactivité Souris
Kits with alternative reactivity to:
(21), (20), (20), (12), (9), (9), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1)
Type de méthode Sandwich ELISA
Gamme de detection 46.9-3000 pg/mL
Seuil minimal de détection 46.9 pg/mL
Application ELISA
Pubmed 1 référence disponible
Fournisseur Connectez-vous pour afficher

Détail du produit EPO ELISA Kit

Détail du antigène Information d'application Stockage References for EPO Kit ELISA (ABIN921054) Images
Fonction Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse EPO
Marque PicoKine™
Type d'échantillon Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate
Méthode de détection Colorimetric
Analytical Method Quantitative
Specificité Expression system for standard: NSO
Immunogen sequence: A27-R192
Réactivité croisée (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensibilité <15pg/mL
Matériel non inclus Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogène Expression system for standard: NSO
Immunogen sequence: A27-R192

Détail du antigène

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Antigène
Autre désignation EPO (EPO ELISA Kit Extrait)
Sujet

Protein Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

Synonyms: Erythropoietin,Epo,

Full Gene Name: Erythropoietin

Cellular Localisation: Secreted.
ID gène 13856
UniProt P07321
Domaine de recherche Angiogenesis, Cancer, Hematopoietic Progenitors, Organogenesis
Pathways Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity

Information d'application

Détail du produit EPO ELISA Kit Détail du antigène Stockage References for EPO Kit ELISA (ABIN921054) Images Haut de la page
Indications d'application Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Commentaires

Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Plaque Pre-coated
Protocole mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(NSO, A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.
Procédure de l'essai Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.
Précision du teste
  • Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 10.13, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 457, Standard deviation: 17.4, CV(%): 3.8
  • Sample 3: n=16, Mean(pg/ml): 1046, Standard deviation: 55.4, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 246, Standard deviation: 23.12, CV(%): 9.4
  • Sample 2: n=24, Mean(pg/ml): 486, Standard deviation: 36.45, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 1118, Standard deviation: 43.6, CV(%): 3.9
Restrictions For Research Use only

Stockage

Détail du produit EPO ELISA Kit Détail du antigène Information d'application References for EPO Kit ELISA (ABIN921054) Images Haut de la page
Conseil sur la manipulation Avoid multiple freeze-thaw cycles.
Stock -20 °C,4 °C
Stockage commentaire Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Date de péremption 12 months

References for EPO Kit ELISA (ABIN921054)

Détail du produit EPO ELISA Kit Détail du antigène Information d'application Stockage Images Haut de la page
Background publications

Sirén, Fratelli, Brines, Goemans, Casagrande, Lewczuk, Keenan, Gleiter, Pasquali, Capobianco, Mennini, Heumann, Cerami, Ehrenreich, Ghezzi: "Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 7, pp. 4044-9, 2001

Images

Détail du produit EPO ELISA Kit Détail du antigène Information d'application Stockage References for EPO Kit ELISA (ABIN921054) Haut de la page
Supplier Images
ELISA image for Erythropoietin (EPO) ELISA Kit (ABIN921054) Mouse EPO PicoKine ELISA Kit standard curve