anti-Aldolase (ALD) Anticorps

May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. De plus, nous expédions Aldolase Kits (10) et et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID
ALD  
ALD  
ALD  
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Top anti-Aldolase Anticorps sur anticorps-enligne.fr

Showing 10 out of 47 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Fournisseur Livraison Prix Détails
Lapin Chèvre Inconjugué ELISA IgG purified antibody to rabbit muscle aldolase (100-1141, 200-1141 and 200-1341) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. Western Blot of Goat anti-Aldolase Antibody. Lane 1: Hela lysate. Lane 2: HEK293 lysate. Lane 3: Jurkat lysate. Load: 25 µg per lane. Primary antibody: Goat anti-Aldolase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP Dk-a-Gt IgG secondary antibody at 1:40,000 for 30 min at RT. Block: ABIN925618 for 30 min at RT. Predicted/Observed size: 39 kDa, 41 kDa for Aldolase. 100 μg Connectez-vous pour afficher 5 to 7 Days
$583.50
Détails
Humain Lapin Inconjugué IF (p), IHC (p), WB Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Aldolase A Polyclonal Antibody, Unconjugated (ABIN739800) at 1:200 followed by conjugation to the secondary antibody and DAB staining 100 μL Connectez-vous pour afficher 3 to 7 Days
$318.45
Détails
Humain Lapin Inconjugué IF, IHC (p), WB Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody at 1:1000 at each lane. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Connectez-vous pour afficher 2 to 3 Days
$515.63
Détails
Lapin Chèvre HRP ELISA, WB IgG purified antibody to rabbit muscle aldolase (100-1141, 200-1141 and 200-1341) was used at a 1:1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. 100 μg Connectez-vous pour afficher 5 to 7 Days
$583.50
Détails
Lapin Chèvre Biotin IP, ELISA, WB Anti aldolase antibody – Immunoprecipitation and Western Blot. 300 µl aliquots of whole anti-aldolase antiserum  were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH.  90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.  Gel was stained, destained and imaged(see attached) using standard protocols.  Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase.  Additional higher and lower molecular weight bands correspond to serum proteins. Anti aldolase antibody– Immunoprecipitation- Immunoprecipitation was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS.  Antibody/Antigen mixture was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH.  90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.  Gel was stained, destained and imaged(see attached) using standard protocols.  Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase.  Additional higher and lower molecular weight bands correspond to serum proteins. 100 μg Connectez-vous pour afficher 5 to 7 Days
$583.50
Détails
Humain Lapin Cy5.5 IF (p)   100 μL Connectez-vous pour afficher 14 to 21 Days
$417.45
Détails
Humain Lapin FITC IF (p)   100 μL Connectez-vous pour afficher 14 to 21 Days
$417.45
Détails
Humain Lapin HRP IHC (p), WB   100 μL Connectez-vous pour afficher 14 to 21 Days
$417.45
Détails
Humain Lapin Cy3 IF (p)   100 μL Connectez-vous pour afficher 14 to 21 Days
$417.45
Détails
Humain Lapin Cy7 IF (p)   100 μL Connectez-vous pour afficher 14 to 21 Days
$417.45
Détails

anti-Aldolase Anticorps mieux référencés

  1. Human Polyclonal Aldolase Primary Antibody pour IF (p), IHC (p) - ABIN739800 : Yao, Zhuang, Du, Cheng, Yang, Guan, Hu, Zhu, Christine, Shi, Hua: Role of Fas-associated death domain-containing protein (FADD) phosphorylation in regulating glucose homeostasis: from proteomic discovery to physiological validation. dans Molecular & cellular proteomics : MCP 2013 (PubMed)

Plus d’anticorps contre Aldolase partenaires d’interaction

Fruit Fly (Drosophila melanogaster) Aldolase (ALD) interaction partners

  1. This study demonistrated that a mutation in Drosophila Aldolase causes temperature-sensitive paralysis, shortened lifespan, and neurodegeneration.

Aldolase (ALD) profil antigène

Profil protéine

May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. Protein acts on two substrates fructose 1,6-bisphosphate and fructose 1-phosphate (like other class I aldolases).

Gene names and symbols associated with anti-Aldolase (ALD) Anticorps

  • Aldolase (Ald) anticorps
  • ald anticorps
  • BcDNA:LP07735 anticorps
  • CG6058 anticorps
  • Dmel\\CG6058 anticorps
  • FBA anticorps
  • T4 anticorps
  • T6 anticorps
  • T7 anticorps
  • T12 anticorps

Protein level used designations for anti-Aldolase (ALD) Anticorps

Ald-PB , Ald-PC , Ald-PD , Ald-PE , Ald-PH , Ald-PI , Ald-PJ , Ald-PK , Ald-PL , CG6058-PB , CG6058-PC , CG6058-PD , CG6058-PE , CG6058-PH , CG6058-PI , CG6058-PJ , CG6058-PK , CG6058-PL , aldolase , fructose 1,6 biphosphate-aldolase A

GENE ID SPECIES
43183 Drosophila melanogaster
Fournisseurs de qualité sélectionnés pour anti-Aldolase (ALD) Anticorps
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