anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) Anticorps

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. De plus, nous expédions CDC20 Protéines (7) et et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID UniProt
CDC20 991 Q12834
CDC20 107995 Q9JJ66
CDC20 64515 Q62623
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Top anti-CDC20 Anticorps sur anticorps-enligne.fr

Showing 10 out of 151 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Fournisseur Livraison Prix Détails
Humain Lapin Inconjugué IHC (p), WB Western blot analysis of Cdc20 using anti-Cdc20 antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.  Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc20 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc20 at approximately 55KD. The expected band size for Cdc20 is at 55KD. IHC analysis of Cdc20 using anti-Cdc20 antibody .Cdc20 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cdc20 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Connectez-vous pour afficher 4 to 6 Days
$280.00
Détails
Boeuf (Vache) Lapin Inconjugué IHC, WB WB Suggested Anti-CDC20 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:62500  Positive Control:  Hela cell lysate CDC20 is supported by BioGPS gene expression data to be expressed in HeLa 100 μL Connectez-vous pour afficher 2 to 3 Days
$289.00
Détails
Humain Lapin Inconjugué EIA, IP, WB Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto- tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. 0.1 mL Connectez-vous pour afficher 6 to 8 Days
$594.00
Détails
Humain Lapin Inconjugué WB Western blot analysis of CDC20 expression in Raji (A), HEK293T (B), HL60 (C) whole cell lysates. 200 μL Connectez-vous pour afficher 13 to 14 Days
$487.50
Détails
Humain Lapin Inconjugué IF, IHC, WB Western blot analysis of extracts of various cell lines, using CDC20 antibody. 100 μL Connectez-vous pour afficher 11 to 13 Days
$366.77
Détails
Humain Lapin Inconjugué ELISA, WB Western blot analysis of extracts from Jurkat/HeLa cells, using p55CDC Antibody. The lane on the right is treated with the synthesized peptide. 100 μg Connectez-vous pour afficher 2 to 3 Days
$302.50
Détails
Humain Lapin Inconjugué ELISA, IHC, WB Western Blot (WB) analysis of specific cells using Cdc20 Polyclonal Antibody. 100 μL Connectez-vous pour afficher Disponible
$363.46
Détails
Roussette (Chauve-souris) Lapin Inconjugué IHC, IHC (p), WB Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-CDC20 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other for ... 100 μL Connectez-vous pour afficher 11 to 14 Days
$727.83
Détails
Humain Lapin Inconjugué FACS, WB Western blot analysis of CDC20 Antibody (N-term) (ABIN653082) in Hela cell line lysates (35 µg/lane). CDC20 (arrow) was detected using the purified polyclonal antibody. CDC20 Antibody (N-term) (ABIN653082) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. 400 μL Connectez-vous pour afficher 10 to 11 Days
$385.00
Détails
Humain Lapin Inconjugué ELISA, WB Western blot analysis of Phospho-CDC20 (Thr70) in lysates of Jurkat , using Phospho-CDC20 (Thr70) Antibody(ABIN6270305). 100 μL Connectez-vous pour afficher 11 to 12 Days
$450.00
Détails

anti-CDC20 Anticorps mieux référencés

  1. Human Polyclonal CDC20 Primary Antibody pour ICC, IF - ABIN252981 : Di Fiore, Pines: How cyclin A destruction escapes the spindle assembly checkpoint. dans The Journal of cell biology 2010 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal CDC20 Primary Antibody pour ICC, IF - ABIN252980 : Ahlskog, Björk, Elsing, Aspelin, Kallio, Roos-Mattjus, Sistonen: Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. dans Molecular and cellular biology 2010 (PubMed)
    Show all 2 Pubmed References

  3. Human Polyclonal CDC20 Primary Antibody pour IP, WB - ABIN233786 : Yamamuro, Kano, Naito: Functions of FZR1 and CDC20, activators of the anaphase-promoting complex, during meiotic maturation of swine oocytes. dans Biology of reproduction 2009 (PubMed)

  4. Xenopus laevis Monoclonal CDC20 Primary Antibody pour CyTOF, ELISA - ABIN251088 : Sackton, Dimova, Zeng, Tian, Zhang, Sackton, Meaders, Pfaff, Sigoillot, Yu, Luo, King: Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C. dans Nature 2014 (PubMed)

  5. Mouse (Murine) Polyclonal CDC20 Primary Antibody pour IHC, WB - ABIN3021793 : Chen, Xu, Wang, Zhang, Wang, Xin, Liu, Jiang, Zhang, Zhang: CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G1 phase to initiate a new cell cycle in HeLa cells. dans The Journal of biological chemistry 2017 (PubMed)

Plus d’anticorps contre CDC20 partenaires d’interaction

Human Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. Knockdown of CDC20 enhanced the drug sensitivity of TR cells to Temozolomide.

  2. Results showed that CENPA, CDK1 and CDC20 were highly expressed in lung adenocarcinoma (LAC) tissue with co-expression patterns. Also, the integrated microarray analysis demonstrated that CENPA, CDK1 and CDC20 might serve as novel cluster of prognostic biomarkers for LAC.

  3. our data collectively demonstrated that Cdc20 overexpression facilitates the docetaxel resistant of the castration-resistant prostate cancer cell lines in a Bim-dependent manner

  4. findings suggest that application of the CDC20-M signature may permit more selective use of adjuvant therapies for glioma patients and that dysregulated CDC20-M members may provide a therapeutic vulnerability in glioma.

  5. Study indicated that AURKA, CDC20 and TPX2 are over-expressed in smoking related lung adenocarcinoma tissues and their higher mRNA expression levels have a worse prognosis.

  6. we demonstrate that the CDC20-MAD2 complex could also be formed independently of the SAC. Moreover, in prolonged arrest caused by nocodazole treatment, the overall levels of the CDC20-MAD2 complex are gradually, but significantly, reduced and this is associated with lower levels of cyclin B1, which brings a new insight into the mechanism of mitotic "slippage" of the arrested cells.

  7. Cdc20 may be a promising molecular target for chemotherapy.

  8. Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

  9. Cdc20 functions as an important negative regulator of SMAR1 in higher grades of cancer

  10. Data provide support for the recent structure-based models and functionally dissect three elements of Cdc20 inhibition: sequestration of Cdc20 in the core mitotic checkpoint complex, sufficient at low Cdc20 concentrations; inhibition of a second Cdc20 through the Mad3 C terminus, independent of Mad2 binding to this Cdc20 molecule; and occupancy of the APC/C with full MCC, where Mad3 and Apc15 are involved.

  11. CDC20 gene, related to cell proliferation in protein complex A, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis.

  12. A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A(B56), modulates APC/C(Cdc20) assembly.

  13. c-Myc is a driver when combined with kRas/Akt3 oncogenic signals in gliomagenesis, whereas Cdc20 overexpression is a passenger

  14. The ABBA-KEN-ABBA amino acid motif cassette holds the Mitotic Checkpoint Complex (MCC) onto the Anaphase-Promoting Complex-Cyclosome (APC/C) by binding the two Cdc20 molecules in the MCC-APC/C complex.

  15. It discuses the roles of Cdc20 in SAC signalling and mitotic exit, describe how the integration of traditional approaches with emerging technologies has revealed new details of Cdc20 functions, comment about the potential of Cdc20 as a therapeutic target for the treatment of human malignancies.

  16. Prostate cancer-derived SPOP mutants failed to interact with Cdc20 to promote its degradation. As a result, SPOP-deficient prostate cancer cells with elevated Cdc20 expression became resistant to a pharmacological Cdc20 inhibitor.

  17. High CDC20 expression is associated with metastatic castration-resistant prostate cancer growth and reduces chemosensitivity .

  18. These results provide novel insight into the mechanisms underlying the aberrant capability of NUP98 oncoproteins to interact with APC/C(Cdc20) and to interfere with its function.

  19. In lung adenocarcinoma patients, overexpression of cell division cycle 20 was significantly associated with bigger primary tumor size, higher MKI67 level, higher DNA ploidy level, and poor prognosis.

  20. CDC20 may have a role in carcinoma of the breast, colon, endometrium, and prostate

Xenopus laevis Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. Cdc20 auto-ubiquitylation does not play a major role in terminating Cdc20 activation.

  2. Dephosphorylation of Cdc20 is required for its loading and activation of the APC/C ubiquitin ligase.

  3. A role for the fizzy/cdc20 family of proteins in activation of the APC/C distinct from substrate recruitment is reported.

Mouse (Murine) Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. Cdc20 hypomorphism causes chromatin bridging and chromosome misalignment, revealing a requirement for Cdc20 in efficient sister chromosome separation and chromosome-microtubule attachment.

  2. The physiologically effective threshold level of Cdc20 is high for female meiosis I.

  3. Results indicate that Cdc20 also contributes to post-anaphase activation of the APC/C.

  4. This cell division cycle protein is a key regulator of the cell cycle.

  5. The seven tandem WD motifs of Cdc20 they are required for speriolin binding and for localization of Cdc20 to the centrosomes and nucleus, suggesting that speriolin might regulate or stabilize the folding of Cdc20 during meiosis in spermatogenic cells

  6. Data suggest that Mad2 and BubR1 must cooperate to inhibit Cdc20 activity.

  7. Cdc20 is degraded through two independent degradation signals (degrons), the KEN box and a newly described CRY box.

  8. Cdc20 and securin double mutant embryos could not maintain the metaphase arrest, suggesting a role of securin in preventing mitotic exit

  9. Expression of the cdc20 gene is down-regulated by zif268 in neuronal cells; altered expression of proteasome-regulatory genes following zif268 induction may be a key component of long-lasting CNS plasticity.

  10. Cdc20 is required for the anaphase onset of the first meiosis but not the second meiosis in mouse oocytes

  11. findings suggest a novel function of HSF1 frequently overexpressed in cancer cells, to inhibit APC/C activity by interacting with Cdc20, and to result in aneuploidy development and genomic instability

  12. Data show that the BubR1 N terminus binds to Cdc20 to inhibit APC/C activity in interphase, thereby allowing accumulation of cyclin B in G(2) phase prior to mitosis onset.

  13. Results indicate that the spindle assembly checkpoint-mediated inhibition of Cdc20 is an important tumor-suppressing mechanism.

Pig (Porcine) Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) interaction partners

  1. porcine FZR1 and CDC20 work on the maintenance of meiotic arrest at the first meiotic prophase and on the exit from M1

CDC20 profil antigène

Profil protéine

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.

Gene names and symbols associated with CDC20

  • cell division cycle 20 (cdc20) anticorps
  • cell division cycle 20 (CDC20) anticorps
  • cell division cycle 20 L homeolog (cdc20.L) anticorps
  • cell division cycle 20 (Cdc20) anticorps
  • cell division cycle 20 homolog (S. cerevisiae) (CDC20) anticorps
  • 2310042N09Rik anticorps
  • bA276H19.3 anticorps
  • C87100 anticorps
  • CDC20A anticorps
  • fizzy1 anticorps
  • p55CDC anticorps
  • X-FZY anticorps

Protein level used designations for CDC20

cell division cycle 20 homolog , CDC20 cell division cycle 20 homolog , cell division cycle protein 20 homolog , fizzy1 , mmCdc20 , cell cycle protein p55CDC , p55CDC

GENE ID SPECIES
394542 Xenopus (Silurana) tropicalis
991 Homo sapiens
398044 Xenopus laevis
107995 Mus musculus
64515 Rattus norvegicus
424563 Gallus gallus
482534 Canis lupus familiaris
397379 Sus scrofa
515376 Bos taurus
Fournisseurs de qualité sélectionnés pour anti-CDC20 (CDC20) Anticorps
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