anti-Extra Spindle Poles Like 1 (ESPL1) Anticorps

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. De plus, nous expédions ESPL1 Kits (7) et et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID UniProt
ESPL1 9700 Q14674
ESPL1 105988 P60330
ESPL1 315330  
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Top anti-ESPL1 Anticorps sur anticorps-enligne.fr

Showing 10 out of 183 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Fournisseur Livraison Prix Détails
Humain Lapin Inconjugué IC, IF, IHC, WB Immunofluorescent analysis of Separase (pS801) staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Connectez-vous pour afficher 13 to 14 Days
$575.00
Détails
Humain Lapin Inconjugué EIA, IHC (p) 0.4 mL Connectez-vous pour afficher 6 to 8 Days
$484.00
Détails
Humain Lapin Inconjugué IF, IHC, ELISA, WB Western blot analysis of extracts from 293 cells, treated with EGF 200ng/ml 30', using SEPARASE (Ab-801) Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Ab-801) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Connectez-vous pour afficher 2 to 3 Days
$302.50
Détails
Humain Lapin Inconjugué IC, IF, IHC, WB   200 μL Connectez-vous pour afficher 13 to 14 Days
$487.50
Détails
Humain Lapin Inconjugué IF, IHC, ELISA, WB Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Connectez-vous pour afficher 2 to 3 Days
$302.50
Détails
Humain Lapin Inconjugué ELISA, IF, IHC, WB Western Blot (WB) analysis of specific cells using Separase Polyclonal Antibody. 100 μL Connectez-vous pour afficher Disponible
$363.46
Détails
Humain Lapin Inconjugué ELISA, IF, IHC, WB 100 μL Connectez-vous pour afficher Disponible
$363.46
Détails
Humain Lapin Inconjugué DB, IHC (p) Dot blot analysis of anti-hSeparase-S801 Phospho-specific Pab (ABIN389654) on nitrocellulose membrane.  50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5 µg/mL. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL Connectez-vous pour afficher 10 to 11 Days
$385.00
Détails
Humain Souris Inconjugué IHC (p), ELISA, WB ESPL1 monoclonal antibody (M01), clone 6H6 Western Blot analysis of ESPL1 expression in Hela S3 NE . Immunoperoxidase of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] 100 μg Connectez-vous pour afficher 11 to 12 Days
$440.00
Détails
Humain Lapin Inconjugué ELISA, IF, IHC, IHC (p), WB 50 μL Connectez-vous pour afficher 11 to 14 Days
$484.00
Détails

anti-ESPL1 Anticorps mieux référencés

  1. Human Monoclonal ESPL1 Primary Antibody pour IHC (p), ELISA - ABIN564183 : Zhang, Ge, Meyer, Sethi, Basu, Pradhan, Zhao, Li, Cai, El-Naggar, Baladandayuthapani, Kittrell, Rao, Medina, Pati: Overexpression of Separase induces aneuploidy and mammary tumorigenesis. dans Proceedings of the National Academy of Sciences of the United States of America 2008 (PubMed)
    Show all 7 Pubmed References

  2. Human Monoclonal ESPL1 Primary Antibody pour ICC, IF - ABIN151796 : Chestukhin, DeCaprio: Western blot screening for monoclonal antibodies against human separase. dans Journal of immunological methods 2003 (PubMed)
    Show all 6 Pubmed References

  3. Monoclonal ESPL1 Primary Antibody pour IF - ABIN534070 : Chestukhin, Pfeffer, Milligan, DeCaprio, Pellman: Processing, localization, and requirement of human separase for normal anaphase progression. dans Proceedings of the National Academy of Sciences of the United States of America 2003 (PubMed)
    Show all 4 Pubmed References

  4. Human Polyclonal ESPL1 Primary Antibody pour DB, IHC (p) - ABIN537755 : Waizenegger, Giménez-Abián, Wernic, Peters: Regulation of human separase by securin binding and autocleavage. dans Current biology : CB 2002 (PubMed)
    Show all 3 Pubmed References

  5. Human Polyclonal ESPL1 Primary Antibody pour DB, IHC (p) - ABIN389654 : Haaß, Stehle, Nittka, Giehl, Schrotz-King, Fabarius, Hofmann, Seifarth: The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib. dans PLoS ONE 2012 (PubMed)

  6. Human Monoclonal ESPL1 Primary Antibody pour WB - ABIN151894 : Sun, Kucej, Fan, Yu, Sun, Zou: Separase is recruited to mitotic chromosomes to dissolve sister chromatid cohesion in a DNA-dependent manner. dans Cell 2009 (PubMed)

Plus d’anticorps contre ESPL1 partenaires d’interaction

Human Extra Spindle Poles Like 1 (ESPL1) interaction partners

  1. Separase activity measurement may therefore be useful as a novel additional molecular marker for disease monitoring

  2. Studies identified and characterized the role of separase in mitosis, meiosis, non-canonical roles, its regulation, as a regulator of centriole disengagement, non proteolytic roles, diverse substrates, structural insights, and association of separase with cancer. [review]

  3. High ESP expression is associated with breast cancer.

  4. Proximity mapping of human separase has been presented.

  5. The assay was used to quantify Separase proteolytic activity in leukemic cell lines and peripheral blood samples from leukemia patients.

  6. Recruitment and activation of separase at centrosomes are two distinct steps that do not require microtubules.

  7. Separase protein levels decrease and Separase proteolytic activity increases exclusively in b3a2 p210BCR-ABL-positive cell lines under Imatinib treatment.

  8. Separase is an oncogene whose overexpression induces tumorigenesis, and indicate that Separase overexpression and aberrant nuclear localization are common in many tumor types and may predict outcome in some human malignancies.

  9. High ESPL1 mRNA expression was associated with luminal B breast cancers.

  10. Data indicate that separase is subject to native-state cis/trans isomerization by peptidyl-prolyl-isomerase Pin1.

  11. Mutation of the homologous position in PTTG1 (H(134)) switched PTTG1 from an inhibitor into an activator of ESP1.

  12. By consecutively acting as a protease and a cdk1 inhibitor, separase coordinates two key processes to achieve simultaneous and abrupt separation of sister chromatids.

  13. Kendrin is a novel and crucial substrate for separase (ESPL1) at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis.

  14. SMC3 and separase are upregulated and securin is downregulated in malignant transformation of BEAS-2B cells induced by coal tar pitch smoke extracts.

  15. Results identify a new functional role of securin and separase in the modulation of membrane traffic and protein secretion that implicates regulation of V-ATPase assembly and function.

  16. Plk1-mediated phosphorylation of Cdc6 promotes the interaction of Cdc6 and Cdk1, leading to the attenuation of Cdk1 activity, release of separase, and subsequent anaphase progression.

  17. Data suggest that separase is required for sister chromatid separation during mitosis in human cells, and that securin inhibits separase by blocking the access of substrates to the active site of separase.

  18. Anaphase specific auto-cleavage of separase.

  19. Processing, localization, and requirement of separase for normal anaphase progression.

  20. density map at a resolution of 25 A of negatively stained separase-securin complex

Mouse (Murine) Extra Spindle Poles Like 1 (ESPL1) interaction partners

  1. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway.

  2. Separase-overexpressing mammary cells are not only susceptible to chromosomal missegregation-induced aneuploidy but also other genetic instabilities

  3. With the continual loss of cohesins from chromosomes that occurs throughout the natural reproductive lifespan, tight regulation of separase in oocytes may be particularly important to maintain cohesion and prevent aneuploidy.

  4. Separase act synergistically with loss of p53 in the initiation and progression of B- and T- cell lymphomas

  5. Separase phospho-regulation is critical for genome stability in oogenesis.

  6. Separase phosphorylation act redundantly to prevent sister chromatid separation.

  7. In embryonic fibroblasts, Separase depletion blocks sister chromatid separation but does not prevent other aspects of mitosis, cytokinesis, or chromosome replication.

  8. Proteolytic activity of separase is therefore essential for Rec8's removal from chromosome arms and for chiasma resolution but not for PBE.

  9. inhibitory phosphorylation of separase plays a critical role in the maintenance of sister chromatid cohesion and genome stability in proliferating postmigratory primordial germ cells

  10. These results collectively suggest that Separase is an oncogene, whose overexpression alone in mammary epithelial cells is sufficient to induce aneuploidy and tumorigenesis in a p53 mutant background.

  11. Results describe a new function for the TGF-beta pathway in the regulation of APC to mediate chromatid separation during mitosis.

  12. deregulation of separase may be a significant contributor to infertility in humans.

ESPL1 profil antigène

Profil protéine

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21\; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009

Gene names and symbols associated with ESPL1

  • extra spindle pole bodies like 1, separase (ESPL1) anticorps
  • extra spindle poles like 1 (espl1) anticorps
  • extra spindle pole bodies 1, separase (Espl1) anticorps
  • extra spindle pole bodies like 1, separase (Espl1) anticorps
  • AL024103 anticorps
  • AU045071 anticorps
  • Cerp anticorps
  • ESP1 anticorps
  • mp:zf637-3-001691 anticorps
  • PRCE anticorps
  • SEPA anticorps
  • SSE anticorps

Protein level used designations for ESPL1

caspase-like protein ESPL1 , extra spindle poles like 1 , extra spindle poles-like 1 protein , separin , separin, cysteine protease , cds , separase , cease&desist , zf637-3-001691 , Cut1/ESP1 related protein

GENE ID SPECIES
9700 Homo sapiens
337864 Danio rerio
506740 Bos taurus
105988 Mus musculus
315330 Rattus norvegicus
425536 Gallus gallus
607879 Canis lupus familiaris
100063779 Equus caballus
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