anti-Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2) Anticorps

Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM, Jul 2010].. De plus, nous expédions HCN2 Kits (9) et HCN2 Protéines (6) et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID UniProt
HCN2 610 Q9UL51
HCN2 114244 Q9JKA9
HCN2 15166 O88703
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Top anti-HCN2 Anticorps sur anticorps-enligne.fr

Showing 10 out of 91 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Fournisseur Livraison Prix Détails
Souris Lapin Inconjugué IHC (p), WB Western blot analysis of HCN2 using anti- HCN2 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat brain tissue lysates,   Lane 2: mouse brain tissue lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HCN2 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN2 at approximately 97KD. The expected band size for HCN2 is at 97KD. IHC analysis of HCN2 using anti- HCN2 antibody . HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- HCN2 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Connectez-vous pour afficher 4 to 6 Days
$280.00
Détails
Humain Lapin Inconjugué WB Western blot analysis of HCN2 expression in SKNSH (A) whole cell lysates. 200 μL Connectez-vous pour afficher 13 to 14 Days
$487.50
Détails
Humain Lapin Inconjugué ELISA, WB Western blot analysis of extracts from Jurkat cells, using HCN2 Antibody. The lane on the right is treated with the synthesized peptide. 100 μg Connectez-vous pour afficher 2 to 3 Days
$302.50
Détails
Humain Souris Inconjugué AA, ICC, IF, IHC, IP, WB HCN2 (S71 37), Human hippocampus. HCN2 Rat Brain Membrane WB. 100 μg Connectez-vous pour afficher 3 to 4 Days
$453.70
Détails
Souris Souris Inconjugué ICC, IHC, IP, WB IHC analysis of HCN2 in frozen sections of mouse brain using HCN2 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested. 100 μg Connectez-vous pour afficher 11 to 14 Days
$551.83
Détails
Humain Lapin Inconjugué ELISA, ICC, IF, WB Western blot analysis of HCN2 using COLO205 whole cell lysates ABIN6278577 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod 100 μL Connectez-vous pour afficher 11 to 12 Days
$390.77
Détails
Humain Souris HRP AA, ICC, IF, IHC, IP, WB Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Connectez-vous pour afficher 3 to 4 Days
$499.20
Détails
Humain Souris FITC AA, ICC, IF, IHC, IP, WB Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Connectez-vous pour afficher 3 to 4 Days
$504.40
Détails
Humain Souris Alkaline Phosphatase (AP) AA, ICC, IF, IHC, IP, WB Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Connectez-vous pour afficher 3 to 4 Days
$507.00
Détails
Humain Souris Biotin AA, ICC, IF, IHC, IP, WB Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 . Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 100 μg Connectez-vous pour afficher 3 to 4 Days
$509.60
Détails

anti-HCN2 Anticorps mieux référencés

  1. Mammalian Monoclonal HCN2 Primary Antibody pour ISt, IHC - ABIN1304697 : Buniel, Glazebrook, Ramirez-Navarro, Kunze: Distribution of voltage-gated potassium and hyperpolarization-activated channels in sensory afferent fibers in the rat carotid body. dans The Journal of comparative neurology 2008 (PubMed)
    Show all 15 Pubmed References

Plus d’anticorps contre HCN2 partenaires d’interaction

Human Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2) interaction partners

  1. beta2-adrenoceptors alone strongly enhanced the activity of HCN2 channels, and co-expression of odorant receptors further diversified the HCN2 channel activity.

  2. TRIP8b competes with a portion of the cAMP-binding site or distorts the binding site by making interactions with the binding pocket, thus acting predominantly as a competitive antagonist that inhibits the cyclic-nucleotide dependence of HCN channels.

  3. HCN2 has a role in maturation and processing of the amyloid precursor protein (Montrer APP Anticorps)

  4. promoter methylation status does not appear to be a major determinant of HCN2 expression in normal and adenoid cystic carcinoma ( ACC )tissues. HCN2 hypomethylation is a biomarker of ACC and may play an important role in the carcinogenesis of ACC.

  5. Aged patients with sinus rhythm exhibited significantly higher expression levels of HCN2 and HCN4 channel mRNA and protein (P<0.05), but significantly lower expression levels of miR1 and 133, compared with those of adult patients with sinus rhythm.

  6. Data show that Rab8b (Montrer RAB8B Anticorps)-interacting protein TRIP8b (Montrer PEX5L Anticorps) does not compete with cyclic AMP (Montrer APRT Anticorps) for the same binding region of hyperpolarization activated cyclic nucleotide gated potassium channel 2 (HCN2).

  7. N-glycosylation is not required for HCN2 channels to function.

  8. Thermal hyperalgesia under chronic inflammatory conditions is mediated by HCN2 channels.

  9. Novel HCN2 mutation contributes to febrile seizures by shifting the channel's kinetics in a temperature-dependent manner.

  10. HCN2 polymorphism may play a role in chronic inflammatory periodontitis but not in peri (Montrer PLIN1 Anticorps)-implantitis.

Mouse (Murine) Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2) interaction partners

  1. Together, these results reveal a critical role for HCN2 in regulating ventral tegmental area dopamine neuronal activity and depressive-related behaviors.

  2. the increased intracellular cAMP drives diabetes-associated pain by facilitating HCN2 activation and consequently promoting repetitive firing in primary nociceptive nerve fibers.

  3. The data of this study strongly suggest that cAMP acts by PKA-mediated regulation of HCN2 channel activity and thereby generates nociceptive sensitization.

  4. Data support a model in which the degree of facilitation of opening in the full-length HCN2 channel by a given ligand depends upon its ability to promote oligomerization of the C-linker/cyclic nucleotide-binding domain in solution.

  5. Mouse HCN2-expressing human mesenchymal stem cells are relevant biological models for the generation of biological pacemakers.

  6. the allosteric mechanism of different cyclic nucleotides on the cyclic nucleotide-binding domain of HCN2 and on channel gating, was investigated.

  7. HCN1 (Montrer HCN1 Anticorps), HCN2, and HCN4 (Montrer HCN3 Anticorps) subunits may have distinct physiological roles in the developing hippocampus.

  8. HCN2 channel activity reduces the repolarization reserve of the ventricular action potential and increases ectopic automaticity under pathological conditions such as excessive beta-adrenergic stimulation.

  9. This study demonstrated that Increased expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in reactive astrocytes following ischemia.

  10. fisetin is a partial agonist for HCN2 channels.

HCN2 profil antigène

Profil protéine

Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.

Gene names and symbols associated with HCN2

  • hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (LOC428335) anticorps
  • hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2) anticorps
  • hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (Hcn2) anticorps
  • hyperpolarization-activated, cyclic nucleotide-gated K+ 2 (Hcn2) anticorps
  • BCNG-2 anticorps
  • BCNG2 anticorps
  • HAC-1 anticorps
  • HAC1 anticorps
  • HCN2 anticorps
  • trls anticorps

Protein level used designations for HCN2

hippocampus abundant transcript 1 , hippocampus abundant transcript 1 protein , hyperpolarization activated cyclic nucleotide-gated potassium channel 2 , brain cyclic nucleotide gated channel 2 , brain cyclic nucleotide-gated channel 2 , potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 , BCNG-2 , HAC-1 , hyperpolarization-activated cation channel 1

GENE ID SPECIES
428335 Gallus gallus
100297361 Bos taurus
100540153 Meleagris gallopavo
610 Homo sapiens
114244 Rattus norvegicus
15166 Mus musculus
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