anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Anticorps

displays differential expression after induction by nerve growth factor [RGD, Feb 2006].. De plus, nous expédions NEDD8 Protéines (29) et NEDD8 Kits (6) et beaucoup plus de produits pour cette protéine.

afficher tous les anticorps Gène GeneID UniProt
NEDD8 4738 Q15843
NEDD8 18002 P29595
NEDD8 25490 Q71UE8
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Top anti-NEDD8 Anticorps sur anticorps-enligne.fr

Showing 10 out of 134 products:

Catalogue No. Reactivité Hôte Conjugué Application Images Quantité Fournisseur Livraison Prix Détails
Yeast Lapin Inconjugué EIA, WB Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody, generated by immunization with full-length recombinant yeast Rub1, was tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # 611-703-127) for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche). Other detection systems will yield similar results. 0.5 mg Connectez-vous pour afficher 6 to 8 Days
$610.50
Détails
Humain Lapin Inconjugué IHC (p), WB NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). Western blot analysis of NEDD8 expression in rat testis extract ( Lane 1), mouse thymus extract ( Lane 2), mouse brain extract ( Lane 3), HELA whole cell lysates ( Lane 4) and MCF-7 whole cell lysates ( Lane 5). NEDD8 at 9KD was detected using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody (Catalog #) at 0.5 µg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). 100 μg Connectez-vous pour afficher 4 to 6 Days
$280.00
Détails
Humain Lapin Inconjugué IHC (p), WB Western blot analysis of NEDD8 polyclonal antibody (ABIN388037) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL Connectez-vous pour afficher 10 to 11 Days
$385.00
Détails
Boeuf (Vache) Lapin Inconjugué IP, IHC, WB Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates. 200 μL Connectez-vous pour afficher 13 to 14 Days
$487.50
Détails
Humain Souris Inconjugué ELISA, FACS, ICC, IHC, WB Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng); Dilution: 1/10000 Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)  Dilution: 1/500 - 1/2000 100 μL Connectez-vous pour afficher 8 to 11 Days
$577.50
Détails
Humain Lapin Inconjugué ELISA, ICC, IF, IHC, WB Western blot analysis on Jurkat cell lysate using NEDD8 Antibody,The lane on the left is treated with the antigen-specific peptide. Western blot analysis of extracts from 293, using NEDD8 Antibody. 100 μL Connectez-vous pour afficher 11 to 12 Days
$390.77
Détails
Humain Souris Inconjugué ELISA, FACS, IF, IHC, WB 100 μg Connectez-vous pour afficher 11 to 14 Days
$551.83
Détails
Humain Lapin Inconjugué IHC, ELISA, WB Western blot analysis of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Connectez-vous pour afficher 2 to 3 Days
$302.50
Détails
Humain Lapin Inconjugué EIA, IHC (p), WB 0.4 mL Connectez-vous pour afficher 6 to 8 Days
$390.50
Détails
Humain Souris Inconjugué CyTOF, ELISA, FACS, ICC, IF, IHC, WB Flow Cytometry: NEDD8 Antibody (1A7) [NBP2-37524] - Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red). Western Blot: NEDD8 Antibody (1A7) [NBP2-37524] - Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa) 0.1 mL Connectez-vous pour afficher 7 to 9 Days
$516.98
Détails

anti-NEDD8 Anticorps mieux référencés

  1. Human Polyclonal NEDD8 Primary Antibody pour IHC (p), ELISA - ABIN542757 : Fan, Bigsby, Nephew et al.: The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast ... dans Molecular endocrinology (Baltimore, Md.) 2003 (PubMed)
    Show all 3 Pubmed References

  2. Yeast (Saccharomyces cerevisiae) Polyclonal NEDD8 Primary Antibody pour ELISA, WB - ABIN539585 : del Pozo, Estelle: The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1. dans Proceedings of the National Academy of Sciences of the United States of America 2000 (PubMed)
    Show all 3 Pubmed References

  3. Human Monoclonal NEDD8 Primary Antibody pour ICC, FACS - ABIN969546 : Ohki, Funatsu, Konishi, Chiba: The mechanism of poly-NEDD8 chain formation in vitro. dans Biochemical and biophysical research communications 2009 (PubMed)

Plus d’anticorps contre NEDD8 partenaires d’interaction

Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. Nedd8 binding to Smurf plays important roles in the regulation of cell migration and the BMP and TGFbeta signaling pathways.

  2. NEDD8-mediated neddylation promotes stress granule assembly.

  3. NEDD8 seems to inhibit the Src-mediated phosphorylation of caveolin-1 by modifying the structure of caveolin-1 protein, which blocks the migration of cancer cells. Although the neddylation process is currently regarded as an emerging target for cancer therapy, our results suggest the possibility that the inhibition of neddylation could facilitate cancer invasion or metastasis at least in some types of cancers.

  4. The cellular effects of NEDD8 inhibition can be manipulated based on the p53 status.

  5. Here we report that high expression of neddylation components, NEDD8 and NAE1, are associated with poor survival of Pancreatic ductal adenocarcinoma patients. Blockage of neddylation by MLN4924 significantly sensitizes pancreatic cancer cells to gemcitabine, reduced clonogenic survival, decreased invasion capacity, increased apoptosis and senescence

  6. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

  7. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells.

  8. MKK7 undergoes neddylation in human breast cancer cells

  9. complete blocking of CRLs at a higher inhibitor dose-induced cytotoxicity that was amplified by knockdown of CRL regulator Cand1.

  10. In conclusion, the authors revealed that E3 ligase HDM2 promotes NEDD8 NEDDylation of HBx to enhance HBx stability and chromatin localization, which in turn favors HBx-dependent transcriptional regulation, cell proliferation, and hepatitis B virus-driven tumor growth.

  11. CUL5 neddylation may allosterically tune polyubiquitin chain length and topology.

  12. This study identifies MyD88 as a novel substrate of NEDD8, and demonstrate that MyD88 NEDDylation antagonizes its ubiquitination.

  13. This study provides new knowledge of the CFTR biosynthetic pathway. It suggests that SYVN1 and FBXO2 represent two distinct multiprotein complexes that may degrade DeltaF508-CFTR in airway epithelia and identifies a new role for NEDD8 in regulating DeltaF508-CFTR ubiquitination.

  14. Neddylation of Smurf1 activates its ubiquitin ligase activity and Smurf2 exerts Nedd8 ligase activity. This study provided new clues of Smurf2 activation regulation.

  15. Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8.

  16. findings add novel evidence showing, for what we believe the first time, that NEDD8-mediated neddylation is required for normal human endometrial functions

  17. In endothelial dysfunction, HDAC2 levels were reciprocally regulated by ectopic expression of NEDD8 and the de-NEDDylating enzyme SENP8.

  18. we report that MLN4924 (NEDD8 enzyme inhibitor ) specifically inhibited protein neddylation, inactivated cullin-RING E3 ligase (CRL), the best-known neddylation substrate, and induced the accumulation CRL substrates in lymphoma cells.

  19. E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma

  20. This work sheds new light on the roles of NEDD8 lysines on neddylation cascades and provides a dominant negative mutant for the study of neddylation and its biological functions.

Mouse (Murine) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

  2. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.

  3. neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 is required for its interaction with TRAF6.

  4. Our findings provide mechanistic evidence of the effects of IAP antagonists in combination with NAE inhibitors, and demonstrate the potential of a new combination therapy for cancer.

  5. a role of Nedd8 in regulating caspase-1 activation following inflammasome activation

  6. Nedd8 and its associated E2 carrier protein (Ubc12) and E3-SCF ligase play a key role in regulating the expression and activity of ENaC in alveolar pneumocytes.

  7. a new regulatory mechanism of RCAN1 function

  8. c-Cbl conjugates neural precursor cell-expressed, developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, to TbetaRII at Lys556 and Lys567.

  9. The NEDD8 pathway appears to play an important role in CD4+ T-cell-mediated inflammatory responses

  10. Nedd8 and AhR localize to overlapping areas in the heart and spinal ganglia, raising the possibility that this interaction may play a role in organogenesis

  11. the analysis of expression pattern in the mouse embryo

  12. NEDD8 and ubiquitin function in a redundant manner in controlling receptor endocytic pathways.

  13. SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation

Fruit Fly (Drosophila melanogaster) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) interaction partners

  1. the Cul1-based SCF complex requires Nedd8 modification for the degradation processes of Ci, Arm, and CycE in vivo

  2. Genetic analyses indicate that a balance between neddylation and deneddylation maintained by DEN1 is crucial for animal viability.

NEDD8 profil antigène

Profil protéine

displays differential expression after induction by nerve growth factor

Gene names and symbols associated with NEDD8

  • neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) anticorps
  • neural precursor cell expressed, developmentally down-regulated gene 8 (Nedd8) anticorps
  • neural precursor cell expressed, developmentally down-regulated 8 (Nedd8) anticorps
  • CG10679 gene product from transcript CG10679-RB (Nedd8) anticorps
  • neural precursor cell expressed, developmentally down-regulated 8 (nedd8) anticorps
  • Nedd8 (Nedd8) anticorps
  • ubiquitin-like protein Nedd8 (nedd8) anticorps
  • neural precursor cell expressed, developmentally down-regulated 8 L homeolog (nedd8.L) anticorps
  • CG10679 anticorps
  • DDBDRAFT_0218116 anticorps
  • DDBDRAFT_0238041 anticorps
  • DDB_0218116 anticorps
  • DDB_0238041 anticorps
  • Dmel\\CG10679 anticorps
  • dNEDD8 anticorps
  • GB17785 anticorps
  • NEDD-8 anticorps
  • nedd8 anticorps
  • nedd8.S anticorps
  • Rub1 anticorps
  • si:zc14a17.5 anticorps
  • ubiquitin anticorps

Protein level used designations for NEDD8

neddylin , ubiquitin-like protein Nedd8 , NEDD-8 , NEDD8 , neural precursor cell expressed developmentally down-regulated protein 8 , neural precursor cell expressed, developmentally down-regulated gene 8 , CG10679-PA , Nedd8-PA , neural precursor cell-expressed developmentally downregulated , neural precursor cell expressed, developmentally down-regulated 8 , Nedd8 , NEDD8 protein , neural precursor cell expressed, developmentally down-regulated 8 S homeolog

GENE ID SPECIES
4738 Homo sapiens
18002 Mus musculus
25490 Rattus norvegicus
35151 Drosophila melanogaster
286796 Bos taurus
368667 Danio rerio
480265 Canis lupus familiaris
549727 Xenopus (Silurana) tropicalis
552822 Apis mellifera
715763 Macaca mulatta
8621655 Dictyostelium discoideum AX4
100009008 Oryctolagus cuniculus
100301786 Oncorhynchus mykiss
100528101 Ictalurus punctatus
100520086 Sus scrofa
446966 Xenopus laevis
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