anti-Translin (TSN) Anticorps

Fruit Fly (Drosophila melanogaster) Translin (TSN) interaction partners

1. Findings indicate that the loss of Translin/TRAX complex (dmC3PO) promoted the accumulation of the passenger strand of esi-2.1 (hp-CG4068B) and increased the accumulation of miR-bantam.

2. Study describes the identification of translin (trsn), a highly conserved RNA/DNA binding protein, as essential for starvation-induced sleep suppression. Strikingly, trsn does not appear to regulate energy stores, free glucose levels, or feeding behavior suggesting the sleep phenotype of trsn mutant flies is not a consequence of general metabolic dysfunction or blunted response to starvation.

3. These studies establish that Trax adopts the translin fold, possesses catalytic centers essential for C3PO's endoRNase activity and interacts extensively with translin to form an octameric assembly.

4. Drosophila translin exhibits a stable dimeric state that assembles into a suboctameric (tetramer/hexamer) form and fails to bind ssDNA and RNA targets

5. A translin null mutant was generated and a trax nonsense mutation was isolated. Translin is essential for stabilizing its interaction with trax.

6. Drosophila translin may have roles in neuronal development and behavior analogous to that of mouse translin.

7. Results suggest that the oligomeric status of translin critically influences the DNA binding properties of translin proteins.

8. an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax was purified; C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products

Human Translin (TSN) interaction partners

1. Translin and Trax control telomere-associated transcript levels in human cells in a telomere-specific fashion.

2. results indicate that UPF1 can dissociate miRNAs from their mRNA targets, making the miRNAs susceptible to Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay.

3. These findings indicate that miRNA depletion in dicer deficiency is due to the combined loss of miRNA-generating activity and catabolic function of TN/TX.

4. this study argues against a mechanism by which translin octamers may "dissociate and reassemble" upon RNA binding and report a novel "open"-barrel structure of human translin revealing a feasible DNA/RNA entryway into the cavity.

5. The selectivity of PLCbeta toward certain genes lies in the rate at which the RNA is hydrolyzed by C3PO.

6. The data demonstrates that the configuration of translin and translin-RNA complexes in solution is a dimer of tetramers, which undergoes conformational transitions.

7. Translin B3 motif, corresponding to TRAX B3 residues, contributes towards its DNA binding activity.

8. C3PO endonuclease is an asymmetric octamer barrel consisting of six translin and two TRAX subunits which activates RISC by degrading the Ago2-nicked passenger strand of siRNA.

9. Sedimentation equilibrium studies using fluorescein-labeled single-stranded DNA support a model in which translin forms an annular structure of eight subunits, an oblate ellipsoid that binds single-stranded DNA at chromosomal breakpoints.

10. study of reactivity of two -SH groups and stidu of proximity of Cys-225 residues

11. At high enough concentrations, translin binds duplex DNA by selectively loading from the free ends, which apparently results in clamping of strands at the duplex ends.

12. The core protein of HCV can interact with translin protein. This can partly explain the molecular mechanism for hepatocellular carcinoma and lymphoma caused by HCV

13. TB-RBP post-transcriptionally stabilizes TRAX and both proteins are needed for normal cell proliferation

14. structure of human translin at 2.2 A resolution is reported in space group C222; translin forms a tetramer; this suggests that the multimerization of translin is flexible; the flexibility may be related to the binding to DNA/RNA

15. Results suggest that translin might be involved in the control of recombination at d(GT)n.d(AC)n microsatellites and in telomere maintenance.

16. Translin "chaperones" Trax and forms heteromeric complex that is DNA binding competent

17. Results describe a circular dichroism analysis of complexes formed between human translin and the microsatellite and telomeric oligodeoxynucleotides d(GT)(12) and d(TTAGGG)(5).

18. Human translin exhibits a stable octameric state and binds ssDNA/RNA/dsDNA targets, all of which get attenuated when GTP is added

19. Findings implicate abnormal dendritic trafficking of BDNF mRNA in the pathophysiology of neuropsychiatric disorders linked to the translin G196A mutation.

Mouse (Murine) Translin (TSN) interaction partners

1. Translin plays an intrinsic role in restricting mesenchymal cell proliferation and differentiation

2. Thus, the authors define a new memory mechanism by which learning reverses microRNA-mediated silencing of the novel plasticity protein ACVR1C via translin/trax.

3. These findings indicate that miRNA depletion in dicer deficiency is due to the combined loss of miRNA-generating activity and catabolic function of TN/TX.

4. These in vivo and in vitro findings of this study indicated that dendritic trafficking of BDNF mRNA can be mediated by both translin-dependent and -independent mechanisms.

5. trax acts to regulate translin's RNA and DNA binding affinities as part of a cellular RNA trafficking mechanism.

6. TB-RBP is involved in the posttranscriptional regulation of Gapds gene expression during spermiogenesis.

7. TB-RBP-null male mice were fertile and sired offspring but had abnormal seminiferous tubules and reduced sperm counts. Null female mice were subfertile, had reduced litter sizes; analysis of brain RNA from null mice revealed an altered gene expression

8. TB-RBP-mouse ribonucleoprotein complex transports a group of specific CREM-regulated mRNAs in mammalian male postmeiotic germ cells.

9. TB-RBP post-transcriptionally stabilizes TRAX and both proteins are needed for normal cell proliferation

10. subcellular locations of TB-RBP and TRAX in male germ cells are modulated by the relative ratios of TRAX and TB-RBP

11. During a search for RNA-induced silencing complex (RISC) proteins that directly interact with small interference RNA, the authors identify TB-RBP in NIH3T3 cell lysate and show that it possesses both single- and double-stranded RNase activities.

12. These studies have, for the first time, identified both mRNAs and a non-coding RNA as TSN targets expressed during meiosis

13. Our results in mice indicate that mutations in translin may contribute to fragile X-like syndromes, mental retardation, attention deficit hyperactivity disorder, epilepsy, and autism spectrum disorders in humans.

14. translin works with SF-1 to augment gene transcription in a DNA-specific fashion

15. Translin contributes to hematopoietic regeneration by acting as a sensor protein for radiation-induced damage

16. The binding of TSN to miR-122a increases its in vivo stability, suggesting an additional posttranscriptional function for TSN

17. Findings implicate abnormal dendritic trafficking of BDNF mRNA in the pathophysiology of neuropsychiatric disorders linked to the translin G196A mutation.

18. GTP binding site mutant and effects on RNA binding and protein binding