anti-Vpx Protein (VPX) Anticorps

Simian Immunodeficiency Virus (SIV) Vpx Protein (VPX) interaction partners

1. Data suggest that a critical role of vpx in vivo/in vitro is to promote degradation of SAMHD1 (SAM domain/HD domain protein 1) in memory CD4+ T-lymphocytes, thereby generating high levels of plasma viremia and induction of immunodeficiency.

2. Vpr and Vpx share a highly similar DCAF1-binding motif, they interact with a different set of residues in DCAF1.

3. SIV vpx is essential for macrophage infection but not for development of simian AIDS.

4. Inhibition of deoxynucleotide hydrolysis by promoting SAMHD1 degradation is not the only mechanism by which Vpx rescues HIV-1 in mature monocyte-derived dendritic cells from the antiviral state.

5. direct down-modulation of Vpx catalytic activity, mediated by the same binding event that leads to SAMHD1 recruitment to the E3 ubiquitin ligase for proteasome-dependent degradation

6. The authors demonstrate that the Wx4Phix2Phix3APhixH motif in SIVmac Vpx is required for both the Vpx-DCAF1 interaction and/or Vpx-mediated degradation of SAMHD1.

7. However, all the vpx mutant viruses were defective for replication in both human and pigtail monocyte-derived macrophages.

8. Vpx induces proteasomal degradation of SAMHD1

9. Vpx relieves the inhibition of lentivirus infection in macrophages by loading SAMHD1 onto the CRL4(DCAF1) E3 ubiquitin ligase, leading to highly efficient proteasome-dependent degradation of the protein

10. This review describes the main functions ascribed to Vpr and Vpx in the context of both viral replication and modulation of host cell biology

11. A single-point mutation in Vpx (H82A) abrogated binding to APOBEC3A and single-round infection of monocytes by HIV-1.

12. Vpx is essential to overcome a block of early infection steps in primary monocytes

13. host cell MAPK signal transduction pathway regulates an early step in SIV infection

14. MacrophageS harbor a potent antiviral restriction; primate lentiviruses have evolved Vpx to counteract this restriction.

15. when transferred in the context of a replication-competent viral clone, Vpx was required for replication in dendritic cells