anti-Humain ATP6AP2 anticorps pour Flow Cytometry

Recommended ATP6AP2 Antibody

Antigène
ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) Anticorps
  • APT6M8-9
  • ATP6IP2
  • ATP6M8-9
  • ELDF10
  • M8-9
  • MRXE
  • MSTP009
  • XMRE
  • (P)RR
  • 5730403E06Rik
  • Atp6ip2
  • tj6
  • wss
  • arcl
  • j6b7
  • stv1
  • tj6m
  • tj6s
  • vph1
  • atp6a2
  • atp6n1d
  • ATP6AP2
  • DKFZp459G0918
  • atp6ap2
  • wu:fj36c03
  • wu:fq15e04
  • zgc:73194
  • ATPase H+ transporting accessory protein 2
  • ATPase, H+ transporting, lysosomal accessory protein 2
  • ATPase H+ transporting V0 subunit d2
  • ATPase H+ transporting V0 subunit a2
  • ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog
  • ATP6AP2
  • Atp6ap2
  • ATP6V0D2
  • atp6v0a2
  • atp6ap2.S
  • atp6ap2
Épitope
C-Term
19
13
11
10
8
8
3
3
2
2
2
1
1
1
1
1
Reactivité
Humain, Souris
114
46
44
6
4
4
4
1
1
1
1
1
1
Hôte
Chèvre
98
18
1
1
Clonalité
Polyclonal
Conjugué
Cet anticorp ATP6AP2 est non-conjugé
8
7
6
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
86
53
34
15
13
9
7
5
5
1
1
Options

Images

N° du produit ABIN185092
$ 429.84
Plus shipping costs $45.00

General

Antigène ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) Anticorps
Épitope C-Term
(19), (13), (11), (10), (8), (8), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivité Humain, Souris
(114), (46), (44), (6), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Hôte Chèvre
(98), (18), (1), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp ATP6AP2 est non-conjugé
(8), (7), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(86), (53), (34), (15), (13), (9), (7), (5), (5), (1), (1)
Pubmed 3 références disponible
Fournisseur

Détail du produit anti-ATP6AP2 anticorps

Détail du antigène ATP6AP2 Information d'application Stockage References for anti-ATP6AP2 antibody (ABIN185092) Images
Fonction ATP6IP2 / Renin receptor
Blocking Peptide Blocking peptide for this product available: ABIN369877
Séquence SIIYRMTNQK IRMD
Homologie Human, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Classe de qualité Verified
Immunogène Peptide with sequence C-SIIYRMTNQKIRMD, from the C Terminus of the protein sequence according to NP_005756.2.

Détail du antigène ATP6AP2

Détail du produit anti-ATP6AP2 anticorps Information d'application Stockage References for anti-ATP6AP2 antibody (ABIN185092) Images Haut de la page
Antigène
Autre désignation ATP6AP2 (ATP6AP2 Antibody Extrait)
Sujet ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9, ELDF10, HT028, MGC99577, MRXE, MSTP009, XMRE, ATPase, H+ transporting, lysosomal interacting protein 2, embryonic liver differentiation factor 10
ID gène 10159
NCBI Accession NP_005756
Pathways ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones

Information d'application

Détail du produit anti-ATP6AP2 anticorps Détail du antigène ATP6AP2 Stockage References for anti-ATP6AP2 antibody (ABIN185092) Images Haut de la page
Indications d'application

DS WB Results: Approx 37 kDa band observed in Mouse Kidney lysates (calculated MW of 39.1 kDa according to NP_081715.1). An additional band of 45 kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2 μg/mL. Primary incubation 1 hour at room temperature.

DS IHC Results: This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan,6(1):9-19, PMID: 27956412.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Stockage

Détail du produit anti-ATP6AP2 anticorps Détail du antigène ATP6AP2 Information d'application References for anti-ATP6AP2 antibody (ABIN185092) Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20 °C.

References for anti-ATP6AP2 antibody (ABIN185092)

Détail du produit anti-ATP6AP2 anticorps Détail du antigène ATP6AP2 Information d'application Stockage Images Haut de la page
Produit citée dans:

Pringle, Zakar, Yates, Mitchell, Hirst, Lumbers: "Molecular evidence of a (pro)renin/ (pro)renin receptor system in human intrauterine tissues in pregnancy and its association with PGHS-2." dans: Journal of the renin-angiotensin-aldosterone system : JRAAS, Vol. 12, Issue 3, pp. 304-10, 2011

Zhu, Yoneda, Demura, Karashima, Usukura, Yamagishi, Takeda: "Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats." dans: Journal of hypertension, Vol. 27, Issue 4, pp. 800-5, 2009

Background publications

Ludwig, Kerscher, Brandt, Pfeiffer, Getlawi, Apps, Schägger: "Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules." dans: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 10939-47, 1998

Images

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Images (Fournisseur)
Western Blotting (WB) image for anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) (C-Term) antibody (ABIN185092) ABIN185092 staining (0.5µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protei...
Western Blotting (WB) image for anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) (C-Term) antibody (ABIN185092) ABIN185092 (2µg/ml) of Mouse Kidney lysate (RIPA buffer, 35µg total protein per lane)...
Flow Cytometry (FACS) image for anti-ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) (C-Term) antibody (ABIN185092) ABIN185092 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line),...