anti-Singe AKT anticorps pour Western Blotting

Recommended AKT Antibody (fourni par: Connectez-vous pour afficher )

Antigène
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Anticorps
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Épitope
pSer473, Internal Region
235
117
114
59
59
50
38
32
28
27
25
21
20
19
18
18
18
14
13
13
13
12
12
12
12
10
10
10
9
8
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus), Singe
1367
798
639
71
56
55
54
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Hôte
Souris
1064
392
11
4
2
1
Clonalité
Monoclonal
Conjugué
Cet anticorp AKT est conjugé à/à la Biotin
55
43
41
40
33
26
15
15
10
10
8
6
6
6
6
6
6
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1262
654
601
290
229
206
136
111
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
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N° du produit ABIN1043752
$ 300.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
3.0617578 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Connectez-vous pour afficher 18F3-H11 4
3.0617578 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Connectez-vous pour afficher 17F6-B11 4
3.0617578 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Connectez-vous pour afficher 18F3-H11 4
3.0617578 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Connectez-vous pour afficher 17F6-B11 0
3.0617578 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Connectez-vous pour afficher 17F6-B11 0
3.0617578 ABIN349626 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Connectez-vous pour afficher 18F3-H11 0
3.0617578 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Connectez-vous pour afficher 18F3-H11 0
3.0617578 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Connectez-vous pour afficher 1F7E10 0
3.0617578 ABIN4880526 ELISA FACS IHC WB Mouse IgG1 AA 1-150 Connectez-vous pour afficher 1F7G2 0
3.0617578 ABIN2707319 IHC WB Rabbit N-Term Connectez-vous pour afficher Polyclonal 0
3.0617578 ABIN2704460 IHC WB Rabbit C-Term, pTyr474 Connectez-vous pour afficher Polyclonal 0
3.0617578 ABIN4880195 ELISA WB Mouse IgG1 Connectez-vous pour afficher 3A3 0
3.0617578 ABIN2715840 FACS IF IHC WB Mouse IgG1 Connectez-vous pour afficher 4E10 1
3.0617578 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Connectez-vous pour afficher 4D6 1
3.0617578 ABIN3182568 ELISA IHC WB Rabbit IgG pTyr474 Connectez-vous pour afficher Polyclonal 0
3.0617578 ABIN2715841 FACS IF IHC WB Mouse IgG1 Connectez-vous pour afficher 4C11 0
3.0617578 ABIN968951 ELISA WB Mouse IgG1 Connectez-vous pour afficher 3A3 3
3.0617578 ABIN2715838 FACS IF IHC WB Mouse IgG1 Connectez-vous pour afficher 4G4 0
3.0617578 ABIN2715836 FACS IF IHC WB Mouse IgG1 Connectez-vous pour afficher 4D6 0
3.0617578 ABIN2715837 FACS IF IHC WB Mouse IgG1 Connectez-vous pour afficher 3E3 0

General

Antigène V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Anticorps
Épitope pSer473, Internal Region
(235), (117), (114), (59), (59), (50), (38), (32), (28), (27), (25), (21), (20), (19), (18), (18), (18), (14), (13), (13), (13), (12), (12), (12), (12), (10), (10), (10), (9), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris, Rat (Rattus), Singe
(1367), (798), (639), (71), (56), (55), (54), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Hôte Souris
(1064), (392), (11), (4), (2), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp AKT est conjugé à/à la Biotin
(55), (43), (41), (40), (33), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1262), (654), (601), (290), (229), (206), (136), (111), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-AKT anticorps

Détail du antigène AKT Information d'application Stockage References for anti-AKT antibody (ABIN1043752) Images
Specificité Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Attributs du produit AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Immunogène Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Immunogen Type: Peptide
Clone 17F6-B11
Isotype IgG1 kappa
Plasmids, Primers & others

Détail du antigène AKT

Détail du produit anti-AKT anticorps Information d'application Stockage References for anti-AKT antibody (ABIN1043752) Images Haut de la page
Antigène
Autre désignation Akt (AKT1 Antibody Extrait)
Sujet AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, phospho AKT antibody, Anti-AKT pS473 Antibody Biotin Conjugated
ID gène 207, 62241011
UniProt P31749
Pathways Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Information d'application

Détail du produit anti-AKT anticorps Détail du antigène AKT Stockage References for anti-AKT antibody (ABIN1043752) Images Haut de la page
Indications d'application Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Commentaires

Gene Name: AKT1

Restrictions For Research Use only

Stockage

Détail du produit anti-AKT anticorps Détail du antigène AKT Information d'application References for anti-AKT antibody (ABIN1043752) Images Haut de la page
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Date de péremption 12 months

References for anti-AKT antibody (ABIN1043752)

Détail du produit anti-AKT anticorps Détail du antigène AKT Information d'application Stockage Images Haut de la page
Produit citée dans:

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" dans: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." dans: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Images

Détail du produit anti-AKT anticorps Détail du antigène AKT Information d'application Stockage References for anti-AKT antibody (ABIN1043752) Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 2)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 3)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 4)
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 5)
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) ELISA of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Antigen: BSA conjuga...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) w...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Immunohistochemistry of mouse Anti-AKT pS473 (MOUSE) Biotin Conjugated  at 40X Tissue...