BAD anticorps (BCL2-Associated Agonist of Cell Death)

Details for Product anti-BAD Antibody No. ABIN1001858
Antigène
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Reactivité
Humain, Souris, Rat (Rattus)
833
507
350
20
19
8
5
4
3
2
2
1
1
1
1
1
Hôte
Lapin
1023
46
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp BAD est non-conjugé
74
67
67
63
62
61
12
12
12
12
12
12
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
552
543
383
374
106
79
45
36
14
13
10
8
6
3
2
1
1
1
1
Options
Immunogène Rabbit polyclol Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad (GenBank accession no. Q92934).
Blocking Peptide Blocking peptide for this product available: ABIN1004255
Isotype IgG
Purification Ion exchange chromatography purified.
Plasmids, Primers & others Plasmids, Primers & others BAD products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Bad (Bcl-2 Family Member) (BAD Antibody Extrait)
Sujet Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation, it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.
Pathways Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events
Indications d'application Bad antibody can be used for detection of Bad by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Stock 4 °C
Date de péremption 12 months
Produit citée dans: Heiser, Labi, Erlacher, Villunger: "The Bcl-2 protein family and its role in the development of neoplastic disease." dans: Experimental gerontology, Vol. 39, Issue 8, pp. 1125-35, 2004 (PubMed).

Cory, Huang, Adams: "The Bcl-2 family: roles in cell survival and oncogenesis." dans: Oncogene, Vol. 22, Issue 53, pp. 8590-607, 2003 (PubMed).

Zhou, Liu, Payne, Lutz, Chittenden: "Growth factors inactivate the cell death promoter BAD by phosphorylation of its BH3 domain on Ser155." dans: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 25046-51, 2000 (PubMed).

Ottilie, Diaz, Horne, Chang, Wang, Wilson, Chang, Weeks, Fritz, Oltersdorf: "Dimerization properties of human BAD. Identification of a BH-3 domain and analysis of its binding to mutant BCL-2 and BCL-XL proteins." dans: The Journal of biological chemistry, Vol. 272, Issue 49, pp. 30866-72, 1998 (PubMed).

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