BIK anticorps (BCL2-Interacting Killer (Apoptosis-Inducing))

Details for Product anti-BIK Antibody No. ABIN1001872
Antigène
  • BIK
  • Biklk
  • Blk
  • Nbk
  • BIP1
  • BP4
  • NBK
  • si:dkey-31f5.6
  • BCL2 interacting killer
  • BCL2-interacting killer
  • BCL2-interacting killer (apoptosis-inducing)
  • BIK
  • Bik
  • bik
Reactivité
Humain, Souris
139
31
9
2
Hôte
Lapin
132
8
Clonalité
Polyclonal
Conjugué
Cet anticorp BIK est non-conjugé
8
5
4
4
4
4
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
114
78
62
29
20
9
8
5
4
2
1
1
1
1
Options
Immunogène BIK antibody was raised against a 15 amino acid peptide from near the amino terminus of human BIK.
Blocking Peptide Blocking peptide for this product available: ABIN1004265
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others BIK products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Bcl-2 Interacting Killer (Apoptosis Inducer NBK) (BIK Antibody Extrait)
Sujet Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b(5) resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.
Pathways Positive Regulation of Endopeptidase Activity
Indications d'application BIK antibody can be used for the detection of BIK by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Stock 4 °C
Date de péremption 12 months
Produit citée dans: Germain, Mathai, McBride, Shore: "Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis." dans: The EMBO journal, Vol. 24, Issue 8, pp. 1546-56, 2005 (PubMed).

Germain, Mathai, Shore: "BH-3-only BIK functions at the endoplasmic reticulum to stimulate cytochrome c release from mitochondria." dans: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18053-60, 2002 (PubMed).

Lockshin, Osborne, Zakeri: "Cell death in the third millennium." dans: Cell death and differentiation, Vol. 7, Issue 1, pp. 2-7, 2000 (PubMed).

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