IKKi/IKKe anticorps (pThr501)

N° du produit ABIN100676

Détail du produit anti-IKKi/IKKe anticorps

Antigène: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Épitope: pThr501

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IKKi/IKKe est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Stérilité: Sterile filtered

Immunogène: IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application: IKKε pT501 antibody is suitable for use in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected.  HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.15 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-IKKi/IKKe (ABIN100676)

DeDiego, Nogales, Martinez-Sobrido, Topham: "Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication." dans: mBio, Vol. 10, Issue 4, (2020) (PubMed).

Sweeney, Mo, Firestein: "Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3." dans: Arthritis and rheumatism, Vol. 56, Issue 3, pp. 743-52, (2007) (PubMed).

Détails sur IKKi/IKKe

Antigène: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Autre désignation: IKK E (IKBKE Produits)

Synonymes: anticorps zgc:100844, anticorps IKBKE, anticorps MGC130907, anticorps IKK-E, anticorps IKK-i, anticorps IKKE, anticorps IKKI, anticorps AW558201, anticorps IKKepsilon, anticorps Ikki, anticorps inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon, anticorps inhibitor of nuclear factor kappa B kinase subunit epsilon, anticorps inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog, anticorps inhibitor of kappaB kinase epsilon, anticorps IKBKE, anticorps ikbke, anticorps ikbke.L, anticorps Ikbke

Sujet: Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity.  IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg.   Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1.  LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis reasearch.
Synonyms: I kappa B kinase epsilon antibody, IkBKE antibody, IKBKE protein antibody, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI, KIAA0151

ID gène: 9641

UniProt: Q9R0T8, Q14164

Pathways: Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades