Chèvre anti-Souris IgA, IgG, IgM (Heavy & Light Chain) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN100805

Détail du produit

Antigène: IgA, IgG, IgM

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG IgA IgM

Réactivité croisée: Souris

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG IgA and IgM whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

ELISA Dilution: 1:500,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: RT,4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG IgA IgM (IgA, IgG, IgM Produits)

Classe de substances: Antibody

Sujet:

Synonyms: Goat Anti Mouse IgG IgA IgM Antibody Biotin Conjugation, Goat Anti-Mouse IgG IgA IgM Biotin conjugated Antibody, Goat Anti-Mouse IgGAM Biotin conjugate

Background: Anti-Mouse IgG IgA IgM Biotin Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.