Chèvre anti-Poulet IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed

Détails pour le produit réf. ABIN101009
Antigène
Épitope
Heavy & Light Chain
1566
134
100
55
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivité
Poulet
355
341
336
232
232
121
113
87
79
73
72
65
61
57
54
49
43
31
27
15
9
8
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Hôte
Chèvre
841
799
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonalité
Polyclonal
Conjugué
FITC
236
218
209
149
147
130
75
49
48
35
31
26
26
26
25
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
15
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogène

Immunogen: Chicken IgG whole molecule

Isotype IgG
Specificité IgG (H&L)
Attributs du produit Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Labeling Ratio 3.7
Antigène
Sujet

Synonyms: goat anti-Chicken IgG Fluorescein Conjugated Antibody, goat anti-Chicken IgG Antibody FITC Conjugation, Chicken Secondary Antibody

Background: Anti-Chicken IgG Fluorescein Antibody generated in goat detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Indications d'application

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Date de péremption 12 months
Images (Fournisseur)
Image no. 1 for Goat anti-Chicken IgG (Heavy & Light Chain) antibody (FITC) - Preadsorbed (ABIN101009) Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody . Lane M: 3 μl Molecular Ladde...