Lapin anti-Cobaye IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed

Détails pour le produit réf. ABIN101298
Antigène
Épitope
Heavy & Light Chain
854
233
202
76
38
13
Reactivité
Cobaye
246
226
173
156
135
80
79
57
54
54
48
41
40
38
25
16
14
13
8
7
7
3
2
1
Hôte
Lapin
626
515
205
49
39
36
10
7
5
3
1
Clonalité
Polyclonal
Conjugué
FITC
184
172
158
135
129
128
50
35
32
30
28
25
22
16
11
9
9
9
9
8
8
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogène

Immunogen: Guinea Pig IgG whole molecule

Isotype IgG
Specificité IgG (H&L)
Attributs du produit Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
Labeling Ratio 2.0
Antigène
Sujet

Synonyms: rabbit Anti-Guinea Pig IgG Antibody Fluorescein Conjugated, rabbit Anti-Guinea Pig IgG Antibody FITC Conjugated

Background: Anti-Guinea Pig IgG Fluorescein Antibody generated in rabbit detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Indications d'application

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Commentaires

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.2 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Agent conservateur Thimerosal (Merthiolate)
Précaution d'utilisation This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Product is photosensitive and should be protected from light.
Stock RT,4 °C,-20 °C
Date de péremption 12 months
Background publications Blakeslee, Baines: "Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG." dans: Journal of immunological methods, Vol. 13, Issue 3-4, pp. 305-20, 1977 (PubMed).