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Chèvre anti-Hamster arménien IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
N° du produit ABIN101379
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 695
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 37
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Hamster arménien
    Hôte
    • 1729
    • 1474
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Chèvre
    Clonalité
    • 3360
    • 167
    • 1
    Polyclonal
    Conjugué
    • 557
    • 409
    • 406
    • 368
    • 297
    • 276
    • 228
    • 121
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 41
    • 39
    • 34
    • 33
    • 33
    • 31
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Alkaline Phosphatase (AP)
    Application
    • 1839
    • 1800
    • 1791
    • 1500
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgG (H&L)
     Réactivité croisée
    Hamster arménien
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose
    Stérilité
    Sterile filtered
    Immunogène

    Immunogen: Armenian Hamster IgG whole molecule

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Hamster Secondary Antibody conjugated to alkaline phosphatase is available in a variety of formats. Anti-IgG Secondary Antibody is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:20,000 - 1:40,000

    Western Blot Dilution: 1:500 - 1:2,500

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Stock
    4 °C
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: goat anti-Armenian Hamster IgG Antibody alkaline phosphatase Conjugation, goat anti-Armenian Hamster IgG alkaline phosphatase Conjugated Antibody, goat Anti-Hamster IgG alk phos Conjugated Secondary Antibody

    Background: Anti-Armenian Hamster IgG Alkaline Phosphatase Antibody generated in goat detects Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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