Lapin anti-Souris IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN101776

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG (H&L)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.

ELISA Dilution: 1:20,000 - 1:400,000

Western Blot Dilution: 1:2,000 - 1:10,000

Commentaires:

Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Référence

Stamatiades, Tremblay, Bohm, Crozet, Bisht, Kao, Coelho, Fan, Yewdell, Davidson, Heeger, Diebold, Nimmerjahn, Geissmann: "Immune Monitoring of Trans-endothelial Transport by Kidney-Resident Macrophages." dans: Cell, Vol. 166, Issue 4, pp. 991-1003, (2016) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: rabbit anti-Mouse IgG Antibody, rabbit anti-mouse IgG heavy and light chain antibody, rb anti-ms IgG heavy and light chain, Anti-Mouse Secondary Antibody

Background: Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.