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Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps - Preadsorbed

ELISA, IHC, WB, FM, IP, LF Polyclonal IgG unconjugated
N° du produit ABIN101983
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 695
    • 672
    • 560
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Lapin
    Hôte
    • 1729
    • 1474
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Chèvre
    Clonalité
    • 3360
    • 167
    • 1
    Polyclonal
    Application
    • 1839
    • 1800
    • 1791
    • 1500
    • 962
    • 933
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 51
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 16
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), Immunoprecipitation (IP), Lateral Flow (LF)
    Specificité
    IgG F(c)
     Réactivité croisée
    Lapin
    Attributs du produit
    Anti-Rabbit IgG antibody generated in goat detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
    Stérilité
    Sterile filtered
    Immunogène

    Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Rabbit IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user

    Immunoprecipitation Dilution: Yes

    ELISA Dilution: 1:10,000 - 1:60,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    IF Microscopy Dilution: Yes

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Date de péremption
    12 months
  • Li, Wu, Yang, Liu, Chen: "Sesquiterpene lactone 6-O-angeloylplenolin reverses vincristine resistance by inhibiting YB-1 nuclear translocation in colon carcinoma cells." dans: Oncology letters, Vol. 15, Issue 6, pp. 9673-9680, (2018) (PubMed).

    Skytt, Toft-Kehler, Brændstrup, Cejvanovic, Gurubaran, Bergersen, Kolko: "Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells." dans: BioMed research international, Vol. 2016, pp. 1087647, (2017) (PubMed).

    Stephen, Higgs, Sheehan, Al Awabdh, López-Doménech, Arancibia-Carcamo, Kittler: "Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 48, pp. 15996-6011, (2015) (PubMed).

  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: goat anti-Rabbit IgG Antibody

    Background: Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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