Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN101994

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Réactivité croisée: Lapin

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with Rabbit IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Antibody Anti-Rabbit IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation, goat anti-Rabbit IgG Alk Phos Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.