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Lapin anti-Mouton IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG
N° du produit ABIN102253
$238.50
Plus shipping costs $45.00
2 mg
local_shipping Destination: Etats-Unis
Envoi sous 1 à 2 jours ouvrables
  • Antigène
    Épitope
    Heavy & Light Chain
    Reactivité
    Mouton
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Conjugué
    FITC
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificité
    IgG (H&L)
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Sheep IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    3.8
  • Indications d'application

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Commentaires

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    2.2 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Product is photosensitive and should be protected from light.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    Sujet

    Synonyms: Rabbit Anti-Sheep IgG fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody FITC Conjugation

    Background: Anti-Sheep IgG (H&L) Fluorescein Antibody generated in rabbit detects reactivity to sheep IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG Antibody is conjugated to Fluorescein.

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