Lapin anti-Mouton IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed Secondary Antibody
- Heavy & Light Chain
- FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- IgG (H&L)
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
Immunogen: Sheep IgG whole molecule
- Labeling Ratio
- Indications d'application
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- For Research Use only
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 2.0 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Product is photosensitive and should be protected from light.
- RT,4 °C,-20 °C
- Date de péremption
- 12 months
Synonyms: Rabbit Anti-Sheep IgG rhodamine Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody TRITC Conjugation
Background: Anti-Sheep IgG (H&L) Rhodamine Antibody generated in rabbit detects reactivity to sheep IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG Antibody is conjugated to Rhodamine.