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Lapin anti-Boeuf (Vache) IgM (Heavy Chain) Anticorps

ELISA, IHC, WB Polyclonal unconjugated
N° du produit ABIN102555
  • Antigène Tous les produits IgM
    IgM
    Épitope
    • 141
    • 117
    • 87
    • 41
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy Chain
    Reactivité
    • 192
    • 134
    • 66
    • 39
    • 32
    • 25
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Boeuf (Vache)
    Hôte
    • 321
    • 187
    • 78
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Lapin
    Clonalité
    • 495
    • 87
    Polyclonal
    Application
    • 335
    • 249
    • 241
    • 209
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 47
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgM µ chain
    Attributs du produit
    Concentration Definition: by Refractometry
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Bovine IgM and Bovine Serum.  No reaction was observed against Bovine IgG F(ab')2  or Bovine IgG F(c).
    Immunogène

    Immunogen: Bovine IgM mu heavy chain

  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    70 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgM
    Abstract
    IgM Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: rabbit anti-Bovine IgM mu chain Antibody, rabbit anti Bovine IgM mu

    Background: Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Bovine IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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