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Chèvre anti-Humain IgM (Heavy & Light Chain) Anticorps (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
N° du produit ABIN102626
  • Antigène Tous les produits IgM
    IgM
    Épitope
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 191
    • 134
    • 66
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 320
    • 188
    • 78
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Chèvre
    Clonalité
    • 495
    • 87
    Polyclonal
    Conjugué
    • 120
    • 77
    • 76
    • 55
    • 36
    • 32
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 335
    • 249
    • 241
    • 209
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 47
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgM µ chain
    Attributs du produit
    Human IgM alpha heavy chain antibody generated in goat detects specifically Human IgM alpha heavy chain. Anti-Human IgM antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Human IgM whole molecule

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-HUMAN IgM (mu chain) (GOAT) Antibody has been assayed against 1.0 μg of Human IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:17,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:150,000 - 1:250,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Aliquot to Avoid repeated freezing and thawing.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgM
    Abstract
    IgM Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Goat Anti-Human IgM Antibody biotin Conjugation

    Background: Anti-Human IgM (mu heavy chain) Biotin generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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