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alpha Tubulin anticorps (AA 427-441) (Biotin)

TUBA1 Reactivité: Humain, Souris, Rat, Poulet, Boeuf (Vache), Chévre, Mouton WB, ELISA Hôte: Lapin Polyclonal Biotin
N° du produit ABIN1043919
  • Antigène Voir toutes alpha Tubulin (TUBA1) Anticorps
    alpha Tubulin (TUBA1)
    Épitope
    • 16
    • 11
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 427-441
    Reactivité
    • 112
    • 77
    • 70
    • 24
    • 18
    • 15
    • 14
    • 13
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Poulet, Boeuf (Vache), Chévre, Mouton
    Hôte
    • 61
    • 59
    • 8
    • 2
    • 2
    • 1
    • 1
    Lapin
    Clonalité
    • 76
    • 55
    • 2
    Polyclonal
    Conjugué
    • 86
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp alpha Tubulin est conjugé à/à la Biotin
    Application
    • 102
    • 44
    • 43
    • 42
    • 38
    • 33
    • 27
    • 17
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificité
    Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.  The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
    Attributs du produit
    Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
    Immunogène
    Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
    Isotype
    IgG
    Top Product
    Discover our top product TUBA1 Anticorps primaire
  • Indications d'application
    Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
    Commentaires

    Gene Name: TUBA1B

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
    Concentration
    0.61 mg/ml
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Date de péremption
    12 months
  • Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." dans: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).

  • Antigène
    alpha Tubulin (TUBA1)
    Autre désignation
    Alpha-Tubulin (TUBA1 Produits)
    Synonymes
    anticorps Tuba1, anticorps K-ALPHA-1, anticorps Tuba-1, anticorps cb944, anticorps fb22g06, anticorps tuba1, anticorps wu:fb22g06, anticorps Calpha1, anticorps tubulin, alpha 1A, anticorps tubulin alpha 1b, anticorps tubulin, alpha 1b, anticorps tubulin, alpha 1B, anticorps Tuba1a, anticorps TUBA1B, anticorps tuba1b, anticorps Tuba1b
    Sujet
    Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
    Synonyms: Loading Control Antibody
    Pathways
    Dynamique des Microtubules
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