Chèvre anti-Hamster arménien IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN1044079

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Hamsters dorés syriens, Hamster arménien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum.

Purification: Preadsorption: Solid phase absorption

Stérilité: Sterile filtered

Immunogène:

Immunogen: Golden Syrian and Armenian Hamster IgG whole molecules

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200-1:1000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Golden Syrian and Armenian Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:2000-1:10,000

Western Blot Dilution: 1:500-1:3000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Stockage commentaire: Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -24 °C or below.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat F(ab')2 Anti-Hamster IgG Antibody Alkaline Phosphatase Conjugation, Goat F(ab')2 Anti-Hamster IgG alk phos Conjugated Antibody, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Alkaline Phosphatase Conjugation, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG alk phos Conjugated Antibody

Background: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Alkaline Phosphatase Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.