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Adhesion G-Protein Coupled Receptor G2 (ADGRG2) (C-Term) anticorps

ADGRG2 Reactivité: Humain, Hamster, Singe, Souris, Rat IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048856
  • Antigène
    Adhesion G-Protein Coupled Receptor G2 (ADGRG2)
    Épitope
    C-Term
    Reactivité
    Humain, Hamster, Singe, Souris, Rat
    Hôte
    • 1
    Lapin
    Clonalité
    • 1
    Polyclonal
    Conjugué
    • 1
    Inconjugué
    Application
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human GPR64. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Opossum (100%) Bovine, Panda, Rabbit (95%) Dog, Bat, Horse (90%) Pig, Platypus (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 20 amino acid peptide from C-terminus of human GPR64. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Opossum (100%), Bovine, Panda, Rabbit (95%), Dog, Bat, Horse (90%), Pig, Platypus (80%).

    Type of Immunogen: Synthetic peptide
  • Indications d'application
    Approved: IHC, IHC-P (5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    Adhesion G-Protein Coupled Receptor G2 (ADGRG2)
    Autre désignation
    ADGRG2 / GPR64
    Sujet
    Name/Gene ID: ADGRG2
    Subfamily: Orphan-B
    Family: GPCR

    Synonyms: ADGRG2, Epididymal protein 6, G protein-coupled receptor 64, EDDM6, G-protein coupled receptor 64, GPR64, HE6, TM7LN2
    ID gène
    10149
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