Metabotropic Glutamate Receptor 5 anticorps (C-Term)
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- Antigène Voir toutes Metabotropic Glutamate Receptor 5 (GRM5) Anticorps
- Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))
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Épitope
- C-Term
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Reactivité
- Rat, Humain, Souris, Chien, Singe, Boeuf (Vache), Cheval, Roussette (Chauve-souris)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Metabotropic Glutamate Receptor 5 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human GRM5 / MGLUR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PLEC (45 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%) Platypus (95%) Opossum, Turkey, Chicken, Lizard (90%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 20 amino acid peptide from C-terminus of human GRM5 / MGLUR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%), Platypus (95%), Opossum, Turkey, Chicken, Lizard (90%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product GRM5 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (4 - 13 μg/mL), WB
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))
- Autre désignation
- GRM5 / MGLUR5 (GRM5 Produits)
- Synonymes
- anticorps GPRC1E, anticorps MGLUR5, anticorps mGlu5, anticorps mGluR5, anticorps mGlur5, anticorps 6430542K11Rik, anticorps AI850523, anticorps Glu5R, anticorps Gprc1e, anticorps mGluR5b, anticorps glutamate receptor, metabotropic 5, anticorps glutamate metabotropic receptor 5, anticorps GRM5, anticorps Grm5
- Sujet
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Name/Gene ID: GRM5
Subfamily: Metabotropic glutamate
Family: GPCR
Synonyms: GRM5, GPRC1E, MGLUR5, Mglur5b, MGlu5 - ID gène
- 2915
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity
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