KCNH2 anticorps (Internal Region)

N° du produit ABIN1048985

Détail du produit anti-KCNH2 anticorps

Antigène: KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))

Épitope: Internal Region

Reactivité: Humain, Rat, Souris, Chien, Lapin, Boeuf (Vache), Cheval, Singe, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KCNH2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human KCNH2 / Kv11.1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Bat, Guinea pig (94%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from internal region of human KCNH2 / Kv11.1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Bat, Guinea pig (94%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur KCNH2

Antigène: KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))

Autre désignation: KCNH2 / HERG (KCNH2 Produits)

Synonymes: anticorps ERG1, anticorps HERG, anticorps HERG1, anticorps Kv11.1, anticorps LQT2, anticorps SQT1, anticorps erg, anticorps KCNH2, anticorps ERG, anticorps gp-erg, anticorps cerg, anticorps derg, anticorps erg1, anticorps AI326795, anticorps LQT, anticorps Lqt2, anticorps M-erg, anticorps Merg1, anticorps merg1a, anticorps merg1b, anticorps potassium voltage-gated channel subfamily H member 2, anticorps potassium voltage-gated channel, subfamily H (eag-related), member 6a, anticorps potassium voltage-gated channel, subfamily H (eag-related), member 2, anticorps potassium voltage-gated channel subfamily H member 6, anticorps KCNH2, anticorps kcnh6a, anticorps Kcnh2, anticorps KCNH6

Sujet: Name/Gene ID: KCNH2
Subfamily: Potassium channel - Eag-related
Family: Ion Channel

Synonyms: KCNH2, Eag homolog, HERG-1, ERG1, HERG, KV11.1, M-erg1, H-ERG, HERG1, SQT1, LQT2, Merg1, Eag-related protein 1, ERG-1

ID gène: 3757