NR1D2 anticorps (N-Term)
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- Antigène Voir toutes NR1D2 Anticorps
- NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))
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Épitope
- N-Term
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NR1D2 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human NR1D2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (94%) Bovine, Bat, Horse (88%) Elephant (81%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 16 amino acid peptide from N-terminus of human NR1D2. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (94%), Bovine, Bat, Horse (88%), Elephant (81%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR1D2 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Antigen retrieval requires Proteinase K treatment. - Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))
- Autre désignation
- NR1D2 (NR1D2 Produits)
- Synonymes
- anticorps BD73, anticorps EAR-1R, anticorps RVR, anticorps HZF-2, anticorps Rev-erb, anticorps REV-ERBB, anticorps NR1D2, anticorps rvr, anticorps bd73, anticorps hzf2, anticorps ear-1r, anticorps hs.37288, anticorps NR1D2-A, anticorps Reverbbeta-A, anticorps reverbb1, anticorps reverbb, anticorps reverbb2, anticorps zgc:92019, anticorps nuclear receptor subfamily 1 group D member 2, anticorps nuclear receptor subfamily 1, group D, member 2, anticorps nuclear receptor subfamily 1, group D, member 2a, anticorps nuclear receptor subfamily 1, group D, member 2b, anticorps NR1D2, anticorps Nr1d2, anticorps nr1d2, anticorps nr1d2a, anticorps nr1d2b
- Sujet
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Name/Gene ID: NR1D2
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: NR1D2, EAR-1R, HZF2, Nuclear receptor rvr, Rev-erbabeta, Rev-erb-beta, Rev-erba beta, RVR, BD73, Nuclear receptor ear-1r - ID gène
- 9975
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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