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NR4A3 anticorps (Internal Region)

NR4A3 Reactivité: Humain, Lapin, Boeuf (Vache), Porc, Chien IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049141
  • Antigène Voir toutes NR4A3 Anticorps
    NR4A3 (Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3))
    Épitope
    • 16
    • 15
    • 13
    • 10
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 101
    • 48
    • 15
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    Humain, Lapin, Boeuf (Vache), Porc, Chien
    Hôte
    • 109
    • 9
    Lapin
    Clonalité
    • 110
    • 8
    Polyclonal
    Conjugué
    • 54
    • 9
    • 9
    • 9
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp NR4A3 est non-conjugé
    Application
    • 111
    • 59
    • 26
    • 26
    • 15
    • 14
    • 14
    • 6
    • 5
    • 4
    • 3
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human NR4A3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Dog, Elephant, Panda, Rabbit, Pig, Platypus (100%) Marmoset, Mouse, Rat, Bat, Hamster, Opossum, Turkey, Chicken (94%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 17 amino acid peptide from internal region of human NR4A3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Dog, Elephant, Panda, Rabbit, Pig, Platypus (100%), Marmoset, Mouse, Rat, Bat, Hamster, Opossum, Turkey, Chicken (94%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product NR4A3 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    NR4A3 (Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3))
    Autre désignation
    Nor-1 / NR4A3 (NR4A3 Produits)
    Synonymes
    anticorps CHN, anticorps CSMF, anticorps MINOR, anticorps NOR1, anticorps TEC, anticorps AI573420, anticorps NOR-1, anticorps Nor1, anticorps NOR-2, anticorps NR4A3, anticorps Nr4a3, anticorps nuclear receptor subfamily 4 group A member 3, anticorps nuclear receptor subfamily 4, group A, member 3, anticorps NR4A3, anticorps Nr4a3, anticorps nr4a3
    Sujet
    Name/Gene ID: NR4A3
    Subfamily: NR4 Nerve growth factor-like
    Family: NHR

    Synonyms: NR4A3, CHN, MINOR, NOR1, Neuron-derived orphan receptor, Nuclear hormone receptor NOR-1, Nor-1, CSMF, Orphan receptor nor1, TEC receptor
    ID gène
    8013
    Pathways
    Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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