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P2RX3 anticorps (C-Term)

P2RX3 Reactivité: Humain, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049196
  • Antigène Voir toutes P2RX3 Anticorps
    P2RX3 (Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3))
    Épitope
    • 16
    • 12
    • 8
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 45
    • 16
    • 13
    • 13
    • 2
    • 1
    Humain, Singe
    Hôte
    • 49
    • 1
    Lapin
    Clonalité
    • 50
    Polyclonal
    Conjugué
    • 21
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp P2RX3 est non-conjugé
    Application
    • 42
    • 21
    • 15
    • 13
    • 13
    • 11
    • 8
    • 4
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human P2RX3 / P2X3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Bat, Rabbit, Opossum (93%) Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse (87%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 15 amino acid peptide from C-terminal cytoplasmic domain of human P2RX3 / P2X3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Bat, Rabbit, Opossum (93%), Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse (87%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product P2RX3 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Antigen retrieval requires Proteinase K treatment.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    P2RX3 (Purinergic Receptor P2x, Ligand-Gated Ion Channel, 3 (P2RX3))
    Autre désignation
    P2RX3 / P2X3 (P2RX3 Produits)
    Synonymes
    anticorps P2X3, anticorps 4930513E20Rik, anticorps P2RX3, anticorps fb99b08, anticorps p2rx3, anticorps p2x3, anticorps wu:fb99b08, anticorps zP2X3, anticorps zfP2X3, anticorps p2xr3.2, anticorps zP2X3.2, anticorps zgc:110486, anticorps purinergic receptor P2X 3, anticorps purinergic receptor P2X, ligand-gated ion channel, 3, anticorps purinergic receptor P2X, ligand-gated ion channel, 3a, anticorps purinergic receptor P2X, ligand-gated ion channel, 3b, anticorps P2RX3, anticorps P2rx3, anticorps p2rx3a, anticorps p2rx3b
    Sujet
    Name/Gene ID: P2RX3
    Subfamily: P2X receptor
    Family: Ion Channel

    Synonyms: P2RX3, ATP receptor, p2X3, p2X3 purinoceptor, p2X purinoceptor 3, Purinergic receptor P2X3, Purinoceptor P2X3, Purinergic receptor, p2RX3, p2X receptor 3, p2X receptor, subunit 3
    ID gène
    5024
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