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Prokineticin Receptor 1 anticorps (N-Term)

PROKR1 Reactivité: Humain WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049243
  • Antigène Voir toutes Prokineticin Receptor 1 (PROKR1) Anticorps
    Prokineticin Receptor 1 (PROKR1)
    Épitope
    • 9
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 28
    • 13
    • 12
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 27
    • 2
    Lapin
    Clonalité
    • 27
    • 2
    Polyclonal
    Conjugué
    • 18
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Prokineticin Receptor 1 est non-conjugé
    Application
    • 11
    • 11
    • 5
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human PROKR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (93%) Elephant, Bovine (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 15 amino acid peptide from N-terminal extracellular domain of human PROKR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (93%), Elephant, Bovine (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product PROKR1 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (7 - 16 μg/mL), WB
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    Prokineticin Receptor 1 (PROKR1)
    Autre désignation
    PROKR1 (PROKR1 Produits)
    Synonymes
    anticorps EG-VEGFR1, anticorps Gpr73, anticorps Pkr1, anticorps GPR73, anticorps GPR73a, anticorps PKR1, anticorps ZAQ, anticorps PROKR1, anticorps prokineticin receptor 1, anticorps Prokr1, anticorps PROKR1
    Sujet
    Name/Gene ID: PROKR1
    Subfamily: Prokineticin
    Family: GPCR

    Synonyms: PROKR1, G protein-coupled receptor 73, GPCR73A, G protein-coupled receptor ZAQ, G-protein coupled receptor 73, G-protein coupled receptor ZAQ, GPR73, GPR73a, PK-R1, Prokineticin receptor 1, EG-VEGF receptor, PKR1, ZAQ
    ID gène
    10887
    Pathways
    Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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