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PTPRA anticorps (Extracellular Domain)

PTPRA Reactivité: Humain IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049272
  • Antigène Voir toutes PTPRA Anticorps
    PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
    Épitope
    • 25
    • 20
    • 15
    • 8
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivité
    • 78
    • 46
    • 41
    • 15
    • 14
    • 13
    • 13
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 94
    Lapin
    Clonalité
    • 94
    Polyclonal
    Conjugué
    • 30
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp PTPRA est non-conjugé
    Application
    • 68
    • 39
    • 39
    • 25
    • 11
    • 9
    • 7
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HLA-S (59 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Rat (94%) Hamster, Dog, Bovine, Rabbit (88%) Mouse, Panda, Horse (82%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 17 amino acid peptide from extracellular domain of human PTPRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat (94%), Hamster, Dog, Bovine, Rabbit (88%), Mouse, Panda, Horse (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product PTPRA Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (15 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
    Autre désignation
    PTPRA / RPTP-alpha (PTPRA Produits)
    Synonymes
    anticorps PTPRA, anticorps heptp, anticorps hlpr, anticorps hptpa, anticorps hptpalpha, anticorps lrp, anticorps ptpa, anticorps ptprl2, anticorps r-ptp-alpha, anticorps rptpa, anticorps HEPTP, anticorps HLPR, anticorps HPTPA, anticorps HPTPalpha, anticorps LRP, anticorps PTPA, anticorps PTPRL2, anticorps R-PTP-alpha, anticorps RPTPA, anticorps Ptpa, anticorps Ptpalpha, anticorps Rptpalpha, anticorps Rptra, anticorps Rptralpha, anticorps RPTP[a], anticorps zf-RPTP[a], anticorps ptpa-a, anticorps protein tyrosine phosphatase, receptor type A, anticorps protein tyrosine phosphatase, receptor type, A, anticorps protein tyrosine phosphatase, receptor type A S homeolog, anticorps PTPRA, anticorps ptpra, anticorps Ptpra, anticorps ptpra.S
    Sujet
    Name/Gene ID: PTPRA
    Subfamily: R4
    Family: Protein Phosphatase

    Synonyms: PTPRA, HLPR, Hptp alpha, HEPTP, HPTPA, PTPA, Ptpalpha, PTPRL2, PTP alpha, PTPLCA-related phosphatase, R-PTP-alpha, RPTPA, Rptpalpha, Tyrosine phosphatase alpha, RPTP alpha, HPTPalpha, LRP, PTPase-alpha
    ID gène
    5786
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