SLC1A3 anticorps (Cytoplasmic Domain)

N° du produit ABIN1049331

Détail du produit anti-SLC1A3 anticorps

Antigène: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Épitope: Cytoplasmic Domain

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC1A3 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SLC1A3 / GLAST. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Hamster, Elephant, Panda, Horse (93%) Marmoset, Rat, Pig (87%) Dog, Bovine, Rabbit, Opossum, Platypus (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 15 amino acid peptide from cytoplasmic domain of human SLC1A3 / GLAST. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Hamster, Elephant, Panda, Horse (93%), Marmoset, Rat, Pig (87%), Dog, Bovine, Rabbit, Opossum, Platypus (80%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur SLC1A3

Antigène: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Autre désignation: SLC1A3 / GluT-1 / GLAST (SLC1A3 Produits)

Synonymes: anticorps AF001784, anticorps CG3747, anticorps Dglt-1, anticorps Dglut-1, anticorps Dmel\\CG3747, anticorps EAAT, anticorps EAAT1, anticorps dEAAT, anticorps dEAAT1, anticorps dEaat1, anticorps slc1a3, anticorps wu:fb98e05, anticorps wu:fd50b05, anticorps zgc:56661, anticorps zgc:77768, anticorps SLC1A3, anticorps GLAST, anticorps GluT-1, anticorps EA6, anticorps GLAST1, anticorps AI504299, anticorps B430115D02Rik, anticorps Eaat1, anticorps GLAST-1, anticorps GLU-T, anticorps Gmt1, anticorps MGluT1, anticorps Excitatory amino acid transporter 1, anticorps solute carrier family 1 member 3, anticorps excitatory amino acid transporter 1, anticorps solute carrier family 1 (glial high affinity glutamate transporter), member 3a, anticorps solute carrier family 1 (glial high affinity glutamate transporter), member 3, anticorps Eaat1, anticorps SLC1A3, anticorps PTRG_00379, anticorps Tsp_02392, anticorps slc1a3a, anticorps Slc1a3

Sujet: Name/Gene ID: SLC1A3
Subfamily: Sodium:dicarboxylate symporter
Family: Transporter

Synonyms: SLC1A3, EA6, EAAT-1, EAAT1, GLAST, GLAST1, GLAST-1, GLUT-1

ID gène: 6507

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport