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His Tag anticorps (HRP)

WB, ELISA, IHC Hôte: Souris Monoclonal 33D10-D2 HRP
N° du produit ABIN1102043
  • Antigène Voir toutes His Tag Anticorps
    His Tag
    Reactivité
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    Hôte
    • 94
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    Souris
    Clonalité
    • 107
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    Monoclonal
    Conjugué
    • 91
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    Cet anticorp His Tag est conjugé à/à la HRP
    Application
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Séquence
    HHHHHH
    Specificité
    This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
    Purification
    Protein A purified
    Immunogène
    This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

    Type of Immunogen: Hapten
    Clone
    33D10-D2
    Isotype
    IgG
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    Discover our top product His Tag Anticorps primaire
  • Indications d'application
    Approved: ELISA, IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)

    Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    100 μL sterile ddH20
    Concentration
    Lot specific
    Buffer
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.
    Agent conservateur
    Gentamicin sulfate
    Précaution d'utilisation
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    avoid freeze thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Antigène
    His Tag
    Abstract
    His Tag Produits
    Classe de substances
    Tag
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