RFP anticorps

N° du produit ABIN1103975

Détail du produit anti-RFP anticorps

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est non-conjugé

Application: Western Blotting (WB), ELISA

Fonction: Anti-RFP / Red Fluorescent Protein Antibody

Specificité: Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.

Purification: Purified

Stérilité: Sterile filtered

Immunogène: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, WB (1:1000 - 1:3000)

Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles. Store undiluted.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Store undiluted.

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: RFP (RFP Produits)