TJP1 anticorps

N° du produit ABIN1109612

Détail du produit anti-TJP1 anticorps

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp TJP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The antibody recognizes specifically ZO-1 alpha-minus.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat, Canine.

Purification: Protein A

Immunogène: Human peptide antigen corresponding to ten residues at the splice junction

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur TJP1

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Autre désignation: ZO-1 / Tight Junction Protein 1 (TJP1 Produits)

Synonymes: anticorps ZO-1, anticorps ZO1, anticorps zo1, anticorps tight junction protein 1, anticorps tight junction protein ZO-1, anticorps TJP1, anticorps Tjp1, anticorps LOC396567

Sujet: Zona occludens 1 (ZO-1) is an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.Synonyms: Tjp1, Zo1, Zona occludens protein 1, Zonula occludens protein 1

ID gène: 7082

NCBI Accession: NP_003248

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization